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Study of chemical composition of propolis extracts from Tetragonisca angustula (jatai) by mass spectrometry and evaluation of its biological activities

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Mara Junqueira Carneiro
Total Authors: 1
Document type: Master's Dissertation
Defense date:
Advisor: Alexandra Christine Helena Frankland Sawaya

Propolis is a resin produced by bees using plant resins for the protection of the hive, particularly against microorganisms. It is a natural product used by humans since ancient times due to its antiseptic, healing and antipyretic properties. It presents a complex chemical composition, which depends on the botanical composition of the region and the bee species. Geopropolis, cerum or propolis produced by stingless bees is causing interest among researchers. The purpose of this research is to study the chemical composition and biological activities of propolis produced by Tetragonisca angustula, or jataí, from different regions of Brazil. The chemical composition was evaluated by direct insertion mass spectrometry fingerprinting and ultra-high efficiency liquid chromatography mass spectrometry (UHPLC-MS). The antioxidant activity was evaluated by two different methods: DPPH and ORAC. The antimicrobial effect of propolis was evaluated by minimal inhibitory concentration (MIC) and the cytotoxic activity by the MTT test. The chemical composition and biological properties of extracts of Schinus terebinthifolius, a plant in the family Anacardiaceae, were also analyzed. This plant is a source of resin for production propolis by jataí. The results showed that the chemical composition of the all extracts of propolis were similar, not depending the region of collection, and exhibited substances in common with the extract of the plant. However, the biological properties of the extracts were different and the extract of plant was more active than extracts of propolis in all the assays (AU)

FAPESP's process: 15/06215-4 - Study of the chemical variability of Brazilian propolis by mass spectrometry and the identification of compounds with antioxidant and antimicrobial activity
Grantee:Alexandra Christine Helena Frankland Sawaya
Support type: Regular Research Grants