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Human adipose tissue mesenchymal stem cells use in a cartilage repair experimental model

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Author(s):
Alessandro Rozim Zorzi
Total Authors: 1
Document type: Doctoral Thesis
Institution: Universidade Estadual de Campinas, Faculdade de Ciências Médicas
Defense date:
Advisor: João Batista de Miranda
Abstract

Background: Articular cartilage has limited healing capacity. Lesions in this tissue have been diagnosed with increasing frequency. Several methods to treat it have been developed, but repair tissue is different from native hyaline cartilage. Autologous Chondrocyte Implantation (ACI) has been recognized as one of the best strategies in the treatment of articular cartilage lesions nowadays, but one major limitation of this technique is the use of adult autologous chondrocytes, which require a two-time surgery, causes donor site morbidity, require a long cell culture time and could evolve with cell dedifferentiation. Stem cells have been evaluated to overcome these problems. Adipose tissue derived mesenchymal stem cells (AT-MSCs) seem to be a promising cell source. Methods: Thirty knees of fifteen female adult sheep were randomly allocated in three groups: Group 1: scaffold with mesenchymal cells; Group 2: scaffold without cells; Group 3: control group with empty untreated lesions. A ten-millimeter size defect was created in the medial femoral condyle, avoiding penetration in the calcified cartilage and bleeding from the subchondral bone. After six months, animals were euthanized and the knees were examined according to macroscopic and histological ICRS 1 scale (primary outcome). Results: All animals completed follow-up. There were no serious adverse events. Group CELLS presented the highest ICRS 1 score (8.3 ± 3.1 SD) against control groups (SCAFFOLD = 5.6 ± 2.2 SD; EMPTY = 5.2 ± 2.4 SD; p = 0.033). Conclusion: AT-MSCs promoted a better healing of partial thickness lesions in the knee of adult sheep (AU)

FAPESP's process: 11/17596-8 - Articular cartilage repair with Type II collagen scaffold and adipose derived mesenchimal stem cells: an experimental sheep model study
Grantee:Joao Batista de Miranda
Support type: Regular Research Grants