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| Author(s): |
Bárbara Santos Pires da Silva
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2014-04-24 |
| Examining board members: |
José Xavier Neto;
Jorg Kobarg;
Chao Yun Irene Yan
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| Advisor: | José Xavier Neto |
| Abstract | |
It was determined that regulatory elements of the atrial-specific expression of the promoter SMyHC3 are contained in a complex nuclear receptor response element (CNRRE). Cellular transactivation assays indicated certain nuclear receptors (NR) can bind in this region. From these trials, was observed the promoter activation by a nuclear receptor, COUP-TFII. It regulates many biological processes such as angiogenesis and atrial development. Deletion of CNRRE resulted in no activation of the promoter by COUP-TFII, indicating their connection in this region. We also verified that only the ligand binding domain of COUP-TFII is able to activate the promoter, suggesting interaction with other NRs to activate it. A proteomic analysis revealed that most of COUP-TFII partners relates to complexes of transcription regulators and the androgen receptor (AR) signaling pathway. Cell transactivation assays showed that together, COUP - TFII and AR, are able to increase promoter activation. (AU) | |
| FAPESP's process: | 11/15273-7 - The role of nuclear receptors in the atrial specification |
| Grantee: | Bárbara Santos Pires da Silva |
| Support Opportunities: | Scholarships in Brazil - Master |