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Influence of hypertension in periapical lesion phenotype pattern, in osteoclastic differentiation, in tissue response and mineralization capacity of MTA repairing cement

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Christine Men Martins
Total Authors: 1
Document type: Doctoral Thesis
Press: Araçatuba. 2016-04-25.
Institution: Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araçatuba
Defense date:
Advisor: João Eduardo Gomes Filho

Among MTA and its products consequences, it is found the participation in mineralization induction in tissue where it is applied and reduction of inflammation maybe present. High blood pressure is a chronic inflammatory disorder that seems acting negatively on calcium mobilization and bone structures of the body. So it can be inferred that periapical lesion development and its treatment using MTA can be altered by the presence of a hypertensive state. Thus, the objective of this research was to study the influence of hypertension in periapical lesion phenotypic, in osteoclast differentiation, in tissue inflammatory response and mineralization ability of MTA repair cements. For this, work was divided into three articles. Article 1 compared potentials aspects of periapical lesion formation in hypertensive and normotensive conditions. Null hypothesis was high blood pressure does not change the number of differentiated osteoclasts, periapical lesion size and expression of IL1α, IL1β and TNFα inflammatory cytokines in apical periodontitis. Among results, although there was no statistically significant difference between periapical lesion size and inflammatory cytokines expression, hypertensive mice showed a large number of differentiated osteoclasts. Article 2 investigated whether hypertension affect tissue response and mineralization ability of white and grey MTA implanted subcutaneously in rats. Null hypotheses were that high blood pressure did not alter tissue response and mineralization capacity against MTA. Through histological analyzes with Hematoxylin and Eosin and Von Kossa stains and under Polarized Light was observed hypertension exacerbates inflammatory response and decrease mineralization capacity, damaging both tissue repair and mineralization. In turn, article 3 investigated whether hypertension affects white and gray MTA mineralization response when they were implanted subcutaneously in rats through osteoblast biomarkers RUNX-2, OPN and OCN. Null hypothesis was that MTA mineralization ability it is not affected by hypertension. The results pointed to the prejudice of MTA mineralization capacity against hypertension. So, in general, it can be concluded that there is an association between periapical problems and its treatment, wherein hypertension impairs number of osteoclasts and MTA action in inflammatory response and mineralization, thus it may be a detrimental factor for successful of endodontic treatment or retreatment. (AU)

FAPESP's process: 13/09446-1 - Influence of hypertension in tissue response and mineralization capacity of cement repairers MTA Angelus® white and grey and IRM®. Histology and immunohistochemistry analysis in rats.
Grantee:Christine Men Martins
Support Opportunities: Scholarships in Brazil - Doctorate