Effect of light control on sperm characteristics and semen proteomic of red-winged tinamou (Rhynchotus rufescens)

Author(s)

Kelry Mayara Silva

• Document type: Doctoral Thesis
• Press: Botucatu. 2020-09-01.
• Institution: Universidade Estadual Paulista (Unesp). Faculdade de Medicina Veterinária e Zootecnia. Botucatu
• Defense date: 2020-08-20
• Advisor: Fabiana Ferreira de Souza

Abstract

The study aimed to evaluate the seminal characteristics and reproductive breeding length of the red-winged tinamou (Rhynchotus rufescens), breed with natural and artificial light, in order to improve reproductive efficiency to commercial breeding (study I). Also, it was aim to describe the sperm and seminal plasma proteomic of tinamou (estudy II). In the suty I, during breeding season (Setember 2017 to March 2018), 20 males were select from 60 males, regarding the ease of semen collection and sperm production. The selected males were divided into 2 groups, control group (natural light, n = 10) and treated group (16 h light and 8 h darkness, n = 10). Semen was collected two time/week and analyzed for volume, subjective motility, vigor, color, contamination degree, sperm concentration and the morphological defects. The study II was similarly conducted to study I. The semen was collected from 28 males, divided in 2 groups (artificial and natural light). After the collections, the samples was grouped in pools of the each groups and centrifuged to separate seminal plasma (supernatant) and spermatozoa (pellet). The samples were prepared to proteomic with triptic digestion and submitted to mass spectrometry. In the study I, there was higher samples number and easiness to collect the sample during in the birds maintained to the artificial light (43 versus 11 from artificial and natural light groups, respectively). The parameters evaluated in semen were also higher (P < 0.001) in birds kept under artificial light. In the study II, 28 proteins were found in the seminal plasma and 58 in the protein extract of the spermatozoa. Keratin and isoforms and catalytic activity and binding were the most frequent proteins and molecular functions, respectively, in samples and groups. In the protein extract of sperm, the proteins alpha-enolase, serum albumin, keratin, type II cytoskeletal cochleal (fragment) and ferritin (fragment) were found in greater abundance in birds kept under natural light. The olfactory receptor proteins (fragment), tail-anchored protein insertion receptor WRB (fragment), keratin, type II cytoskeletal 73 (fragment) and keratin, type II cytoskeletal 75 (fragment) were more abundant in birds with artificial light. The main proteins identified in the seminal plasma were keratin, type II cytoskeletal 75 frag., Keratin, type I cytoskeletal 15, keratin, type I cytoskeletal 17, keratin, type II cytoskeletal 73, keratin, type II cytoskeletal 75, keratin, type II cytoskeletal cochleal , tubulin beta chain (Fragment), glutathione S-transferase Mu 5. In conclusion, the artificial light program can prolong the reproductive season and increase the sperm quality of red-winged tinamou, being indicated for commercial production. Moreover, the main proteins of sperm cells and seminal plasma of redwinged tinamou (Rhynchotus rufescens) were described for first time and were found similar proteins to other species. (AU)

• FAPESP's process: 17/14557-8 - Implantation of artificial insemination program in partridges (Rhynchotus rufescens) based on reproductive performance for commercial production
• Grantee: Kelry Mayara da Silva
• Support Opportunities: Scholarships in Brazil - Doctorate