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| Author(s): |
Valter Henrique Marinho Santos
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | Botucatu. 2016-04-18. |
| Institution: | Universidade Estadual Paulista (Unesp). Instituto de Biociências. Botucatu |
| Defense date: | 2016-02-26 |
| Advisor: | Giuseppina Pace Pereira Lima; Regildo Márcio Gonçalves da Silva |
| Abstract | |
The family Bignoniaceae consists of species that are spread several parts of globe, but in Brazil is most commonly found in areas of Cerrado, Atlantic Forest and Amazon. Within this big family we can highlight the Spathodea campanulata, a species with peculiar characteristic, it has insecticidal and medicinal activity, depending on the plant organ used. Nowadays industries and companies from different fields, they have been investing in studies for the identification and extraction of plant compounds with bioactivity, since they can be beneficial to human health and less harmful to the environment when compared to synthetic chemicals. Due to the present demand for studies of plant species capable of producing bioactive compounds and characteristics of Spathodea campanulata, the study aimed to evaluate the insecticidal potential of specie's nectar on Euschistus heros, Helicoverpa zea e Anticarsia gemmatalis. It also analyzed capacity antioxidant of flowers, leaves and nectar of species by antioxidant tests (ORAC, DPPH e FRAP) and quantification of phenolic compounds and carotenoids by HPLC. The insecticidal tests showed significant toxic activity of nectar on insects Euschistus heros (Fabr.), Helicoverpa zea (Boddie) and Anticarsia gemmatalis (Hübner). Later analysis of the nectar by SDS-PAGE and 2D-PAGE revealed a set of "spots" that can be proteins or even peptides responsible for insecticidal activity. In the oxidant tests, TBARS and Albumin degradation oxidative test, were observed high oxidant activity of undenatured nectar compared to the denatured. In relation to the evaluation of the antioxidant capacity by the methods of FRAP, DPPH and ORAC, the leaf samples had the best results, followed by the samples of flowers and nectars. The same pattern was observed in the test quantification total phenols. In the characterization of bioactive compounds in leaves and flowers, it was possible detect predominantly phenolic compounds (caffeic acid, ferulic acid and rutin), and carotenoids (lutein, zeaxanthin and β-carotene). In both analyses, the samples of leaves and flowers showed higher concentration compared with nectars. Based on the results, we can first conclude that the S.campanulata’s nectar campanulata has insecticidal activity and that this action can be promoted by proteins with direct or indirect toxic effect on insects. The leaves and flowers of this species can serve as an interesting source of secondary metabolites, with phytotherapic and antioxidant activity, of industrial interest in food, pharmaceutical and cosmetology areas. (AU) | |
| FAPESP's process: | 12/24440-7 - Assessing the potential of insecticide nectar Spathodea campanulata (Bignoniaceae) |
| Grantee: | Valter Henrique Marinho dos Santos |
| Support Opportunities: | Scholarships in Brazil - Doctorate |