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| Author(s): |
Regiane Aparecida Ferreira
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | Campinas, SP. |
| Institution: | Universidade Estadual de Campinas (UNICAMP). Faculdade de Ciências Médicas |
| Defense date: | 2016-07-31 |
| Examining board members: |
Fernando Ferreira Costa;
Maria de Fátima Sonati;
Cláudia Vianna Maurer Morelli;
Márcia Regina de Sousa Cossa Wenning;
Anderson Ferreira da Cunha
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| Advisor: | Dulcinéia Martins de Albuquerque; Fernando Ferreira Costa |
| Abstract | |
The beta thalassemia is characterized by partial or complete reduction of the synthesis of beta globin chains. Studies have shown that many transcription factors are involved in regulating the expression of erythroid genes, including the GATA1 complex. Moreover, microRNAs can act as post-transcriptional regulators during erythroid development. In this study, we evaluated the possible relationship of microRNAs both erythroid-specific transcription factors and with the globin genes. Thus, the expression of microRNAs and transcription factors during erythroid differentiation in vitro patients with thalassemia intermediate was realized. Subsequently, miR- 210-3p was selected for subsequent studies and lentiviral vectors were used to inhibit the expression of this miR in K562 and KU812 cells. Down-regulation in the gamma globin and high levels of KLF1 were observed in the inhibited cells. Moreover, in silico analysis showed that miR-210- 3p could target two regions of mKLF1 and we suggest miRNA mediates actions to induce ?-globin expression through KLF1. Our results show, for the first time, a potential target of miR-210-3p and it is related to the hemoglobin switching (AU) | |
| FAPESP's process: | 11/11166-1 - FUNCTIONAL ANALYSIS OF microRNAS AND TRANSCRIPTION FACTORS DURING IN VITRO ERTHTHROID DIFFERENTIATION FROM BETA THALASSEMIA INTERMEDIA PATIENTS |
| Grantee: | Regiane Aparecida Ferreira |
| Support Opportunities: | Scholarships in Brazil - Doctorate |