Effect of a staterin-derived peptide (Stn15pSpS) in the prevention of erosive dental wear of enamel and dentin in vitro

Author(s)

Fabiana Navas Reis

• Document type: Master's Dissertation
• Press: Bauru.
• Institution: Universidade de São Paulo (USP). Faculdade de Odontologia de Bauru (FOB/SDB)
• Defense date: 2023-03-21

Examining board members

Marilia Afonso Rabelo Buzalaf; Alessandra Buhler Borges; Taís Scaramucci Forlin; Linda Wang

• Advisor: Marilia Afonso Rabelo Buzalaf

Abstract

As the etiology of erosive tooth wear (ETW) is multifactorial, there are several possibilities for preventive treatment of the lesions. Preliminary experiments by our group revealed that a statherin-derived peptide from containing the 15 amino acids of the N-terminal region, with serines 2 and 3 phosphorylated (Stn15pSpS), protects enamel against initial erosion. The aim of the present study was to evaluate the effect of solutions or gels containing Stn15pSpS, in different concentrations, on the protection against enamel and dentin erosion in vitro. Bovine enamel specimens were divided into 4 groups (n =15/group), according with the treatment solutions: 1) Deionized water (negative control), 2) Elmex Erosion Protection(TM) (positive control), 3) 1.88 × 10-5 M Stn15pSpS and 4 ) 3.76 × 10-5 M Stn15pSpS. For the gels, bovine enamel and dentin specimens were divided into 2 groups (n = 15 and 18/group for enamel and dentin, respectively) that were treated with chitosan or carboxymethylcellulose (CMC) gels containing Stn15pSpS at 1.88 × 10-5M or 3.76 × 10-5M. Chitosan and CMC gels without active ingredients served as negative controls, while chitosan gel containing 1.23% F (as NaF) and acidulated phosphate fluoride gel (1.23% F) served as positive controls. The solutions and gels were applied on the specimens for 1 min and 4 min, respectively. Stimulated saliva was collected from 3 donors and used to form the acquired pellicle for 2 hours and subsequently applied on specimens, both for gels and solutions. Then, the specimens were submitted to an erosive pH cycling protocol 4 times/day, for 7 days (0.01 M HCl pH 2.0/45 s, artificial saliva/2 h and artificial saliva overnight). The solutions or gels were applied again during pH cycling, 2 times/day for 1 min or 4 min, respectively, after the first and last erosive challenges. Enamel loss (?m) was assessed by contact profilometry. Data of specimens treated with solutions were analyzed by Kruskal-Wallis and Dunn´s tests (p<0.05). Os dados para os espécimes tratados com solução foram analisados pelos testes der Kruskal-Wallis e Dunn (p< 0,05). The best protection against ETW was conferred by Elmex that significantly differed from all the other5 treatments, followed by the Stn15pSpS-containing solutions, regardless of the concentration. Data from gel-treated specimens were analyzed by two-way ANOVA (for chitosan and CMC gels, separately) and Tukey\'s test (p<0.05). Regarding the CMC-based enamel gels, none of the treatments 10 significantly reduced ETW compared to placebo; for dentin, however, gels statherin-containing, regardless of the concentration, significantly reduced ETW. Furthermore, chitosan-based gels, regardless of statherin concentration, were able to protect enamel and dentin against ETW. (AU)

• FAPESP's process: 20/01634-7 - Effect of a statherin-derived peptide (Stn15pSpS) on the prevention of erosive wear of enamel and dentine in vitro
• Grantee: Fabiana Navas Reis
• Support Opportunities: Scholarships in Brazil - Master