Full text
| |
| Author(s): |
Stephanie de Souza Theodoro
Total Authors: 1
|
| Document type: | Doctoral Thesis |
| Press: | Jaboticabal. 2023-07-27. |
| Institution: | Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal |
| Defense date: | 2023-06-07 |
| Advisor: | Aulus Cavalieri Carciofi |
| Abstract | |
The objective was to evaluate and compare the effects of β-glucans extracted from yeast cell wall with that extracted from seaweed on the composition of the intestinal microbiota, fermentation products in the feces and immunological and inflammatory parameters of adult dogs. For the study, a control diet (CON) was supplemented with yeast-extracted B-glucan (BLEV; 0.115%) or seaweed B-glucan: BA15 (0.155% seaweed B-glucan dose); BA31 (0.31% Seaweed B-glucan). 32 dogs were used, 8 per ration. They received the diets for 42 days, with blood and feces collected at the beginning and end of the period for analysis of cytokines, ex vivo production of reactive oxygen and nitrogen intermediates, phagocytic activity of neutrophils and monocytes, C-reactive protein (PCR), parameters lipemics, fecal IgA concentration and calprotectin. In the middle of this period, a digestibility test and a postprandial insulin and glucose curve were performed. At the final time, fresh feces were still collected to evaluate fermentation products. Data were evaluated by analysis of variance and compared by Tukey's test (P<0.05), and when necessary, analysis of covariance was applied. Dogs fed B-A31 showed lower IL-2 than B-LEV and less phagocytosis by neutrophils than the other treatments (P<0.05). Dogs fed B-A31 tended to have higher IL-10 than CON (P=0.095). Consumption of seaweed B-glucan increased nitric oxide production in cell culture and serum CRP (P<0.05). Dogs fed BA15 showed higher apparent digestibility of dry matter, organic matter and gross energy (p<0.05). Dogs fed BLEV tended to have lower mean glucose and shorter time to peak insulin response (p<0.1). The beta diversity of the fecal microbiota of dogs on the BA31 diet differed from the BLEV and BA15 diets. It was concluded that B-glucans from yeast and seaweed showed different pathways of action, as B-glucan from seaweed induced an increase in CRP, monocyte activation measured by increased production of nitric oxide and tended to increase the anti-inflammatory cytokine IL -10, and the BA31 diet showed a greater ability to alter the fecal microbiota. Yeast β-glucan tended to lower mean glucose concentration and anticipate the peak of endogenous insulin action. (AU) | |
| FAPESP's process: | 20/04120-4 - Comparison of the effects in dogs of the consumption of ²-glucans extracted from algae or yeast |
| Grantee: | Stephanie de Souza Theodoro |
| Support Opportunities: | Scholarships in Brazil - Doctorate |