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| Author(s): |
Henrique Luis Piva
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | Ribeirão Preto. |
| Institution: | Universidade de São Paulo (USP). Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (PCARP/BC) |
| Defense date: | 2025-01-23 |
| Examining board members: |
Antonio Claudio Tedesco;
Fernando Lucas Primo;
Valeria Reginatto Spiller;
Roberta Sessa Stilhano Yamaguchi
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| Advisor: | Antonio Claudio Tedesco |
| Abstract | |
This work investigated the development and application of three-dimensional bioprinted scaffolds for tissue regeneration by employing 3D cell culture, 3D printing, and 3D bioprinting techniques, with biomaterials capable of promoting cellular adhesion, proliferation, and differentiation, thereby optimizing processes for various applications in regenerative medicine and advanced therapies. Biocompatible materials such as collagen, sodium alginate, gellan gum, GelMA, chitosan, and curcumin were utilized. In the case of cartilaginous tissues, scaffolds based on alginate and gellan gum resulted in hydrated structures similar to hyaline cartilage, whereas collagen-based scaffolds formed dense cellular networks resembling fibrocartilage. For epithelial tissues, particularly in diabetic wound models, scaffolds incorporating mesenchymal stem cells and curcumin demonstrated efficacy in enhancing wound healing and reducing inflammation. In bone repair models, hydrogels containing GelMA and microparticles associated with cellular spheroids exhibited promising mechanical and biological properties for regenerating critical defects. For the cardiac valve model, collagen scaffolds provided excellent support for cellular growth and showed potential for cardiac valve repair, although additional studies are necessary to confirm clinical viability. Furthermore, the exploration of pre-vascularized scaffolds expanded the range of potential applications. The results indicate significant progress in regenerative therapies, underscoring the potential of 3D bioprinting and the developed biomaterials as viable and innovative solutions for the effective regeneration of damaged tissues, while also contributing to the replacement of animal models with ethical alternatives in research. It is concluded that the techniques employed hold significant potential to transform the field of tissue engineering and clinical practice. (AU) | |
| FAPESP's process: | 20/04009-6 - Development of a novel bioink for 3D printing scaffolds for regeneration of cartilage tissues |
| Grantee: | Henrique Luis Piva |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |