In situ effect of dentifrices associated to CO2 laser in the permeability of eroded root dentine

Author(s)

Renata Siqueira Scatolin

• Document type: Master's Dissertation
• Press: Ribeirão Preto.
• Institution: Universidade de São Paulo (USP). Faculdade de Odontologia de Ribeirão Preto (PCARP/BC)
• Defense date: 2012-01-24

Examining board members

Silmara Aparecida Milori Corona; Maria Cristina Borsatto; Cristiane Tomaz Rocha

• Advisor: Silmara Aparecida Milori Corona

Abstract

Facing the increased prevalence of erosive lesion and the need of preventive means to control painful symptoms related to them, the aim of this study was to evaluate, through a crossover 2 x 2 in situ trial, the effect of a desensitizing dentifrice combined with CO2 laser irradiation to control the permeability of eroded root dentin. Eighty slabs of bovine root dentin (3 x 3 x 2 mm) were subjected to initial erosive challenge (citric acid 0.3%, 2 h), followed by a remineralizing period in artificial saliva (24 h). Specimens were then divided according to dentin treatment: desensitizing dentifrice, desensitizing dentifrice + CO2 laser, fluoride anticaries dentifrice and fluoride anticaries dentifrice + CO2 laser. After a 2-day lead-in period, ten volunteers wore an intraoral palatal appliance containing 4 root dentin slabs, in 2 phases of 5 days each. During the intraoral phase, one side of the appliance was immersed in 0.3% citric acid, and the opposite side in deionized water, four times a day. One hour after the immersions, all specimens were brushed with dentifrice slurry provided by the researcher. At the first phase, half of volunteers brushed the specimens with desensitizing dentifrice, and the other half used the fluoride anticaries dentifrice. After a 7-day wash-out period, volunteers were crossed-over on the dentifrice. Completed each phase the specimens were stained and sectioned to be assessed for permeability through optical microscope. Data were analyzed using ANOVA and no significant difference (p=0,272) was found between the surface treatments performed on bovine root dentin. It can be concluded that fluoride anticaries or desensitizing dentifrice, regardless of association with the CO2 laser irradiation, were able to permeability of eroded root dentin. (AU)

• FAPESP's process: 10/03180-1 - In situ effect of dentifrices associated to CO2 laser in the dentinary permeability after simulation of erosive challenges
• Grantee: Renata Siqueira Scatolin
• Support Opportunities: Scholarships in Brazil - Master