Role of P2X7 receptor in the response of phagocyte... - BV FAPESP
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Role of P2X7 receptor in the response of phagocytes to Plasmodium chabaudi infection.

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Author(s):
Maria Nogueira de Menezes
Total Authors: 1
Document type: Master's Dissertation
Press: São Paulo.
Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
Defense date:
Examining board members:
Maria Regina D'Imperio Lima; Lis Ribeiro do Valle Antonelli; Nancy Starobinas
Advisor: Maria Regina D'Imperio Lima
Abstract

The erythrocyte rupture is a step of the life cycle of Plasmodium in which ATP is released and is recognized by the P2X7. This study of experimental malaria caused by P. chabaudi in P2X7-/- mice showed that these mice are more susceptible to the infection. Either in vitro or ex vivo, the phagocyte cell populations were sensible to extracellular ATP in a P2X7-dependent way and the P. chabaudi infection acted as a component that increases this sensitivity. Phenotypic analysis of the spleen and liver from P2X7-/- mice showed that, excepted for the CD11b+ Ly6G+ population, these mice have a lower number of phagocyte cells when compared to C57BL/6 mice. Furthermore, there was a slight deficiency in phagocyte activation, IFN-g production and number of cells that produce important cytokines as IFN-g, IL-10 and TNF-a in P2X7-/- mice in comparison with C57BL/6 mice. The increased susceptibility of P2X7-/- mice to the P. chabaudi infection is due to a deficient immune response. (AU)

FAPESP's process: 11/14836-8 - Role of P2X7 receptor on activation and death of antigen presenting cells induced by T CD4+ cells of mice with acute and chronic malaria
Grantee:Maria Nogueira de Menezes
Support Opportunities: Scholarships in Brazil - Master