Abstract
The scope of this work is the technical validation of an enzymatic formulation to stabilize LuciStar, an enzyme developed by BIOLINKER in AMBEV Line products. Biolinker's proposal consists in subjecting a luciferase to directed evolution in order to make it functional under the following conditions: pH 2.5-4.5; temperature 3-5 °C; presence of 4-8 % alcohol. These approximate the environme…