- Research Grants
Luis Felipe Santos Mendes studied Biomolecular Physics in the Physics Department of the University of São Paulo and graduated in 2011. For his work with distance measurements using continuous-wave and pulsed electron magnetic resonance applied to proteins involved in calcium-response homeostasis, he was granted and spent three months in the Gunnar Jeschke lab at the ETH-Zurich. As a PhD student in the Faculty of Philosophy, Science and Letters of the University of São Paulo, he was inserted in a structural biology project involving a multitask protein called GRASP, which work as key factors in the Golgi biogenesis and Unconventional Secretory Pathways. During the PhD, he spent one year as guest student in the New Biochemistry Department of the University of Oxford-UK (supervision of Prof. Dr. Anthony Watts) and one month in the Chemistry Department of NCSU (guidance of Prof. Dr. Alex I. Smirnov). He currently works as a postdoctoral fellow at the Physics Department of the University of São Paulo and seeks to elucidate the mechanism of action of soluble (GRASP55 and 65) and integral membrane (p24/TMED family) proteins in the early secretory pathway (linked to the Center for Research in Inflammatory Diseases). Inside this main project, we also started to seek for a explanation of a Metazoa-specific protein paralogy (using GRASP55/GRASP65 as a model case) by going back to the past using Protein Ancestor Sequence Reconstruction. He has experience in classical molecular biology techniques (cloning, subcloning, mutant construction, expression and purification of heterologous soluble/membrane proteins) and a variety of spectroscopic techniques, with special emphasis on Circular Dichroism (conventional and synchrotron-radiation), fluorescence (steady-state and lifetime techniques) and Magnetic Resonances (electronic and nuclear). (Source: Lattes Curriculum)
Each and every living cell evolved in a way where an efficient biomolecular secretion system was created in order to attend its basal biological needs and the way they interact with external systems. In this sense, eukaryotic cells enormously differ from prokaryote ones, possessing an extensive biomembrane system in their intracellular part, where different compartments with specific fu...
The secretion of virulence factors is a critical mechanism for the establishment of criptococose, a disease that along with candidiasis represent opportunistic fungi infection in HIV patients. Criptococose is a disease caused by Cryptococcus neoformans, which appears to be the only pathogen species from the Cryptococcus genus. The virulence of this pathogen seems to be related to the ex...
The current research project is an extension of the FAPESP PhD scholarship hold by Felipe Mendes and is included in a recently approved FAPESP/BBSRC grant (Proc. FAPESP 2015/50366-7) aimed at supporting a formal collaboration between our group and Prof. Anthony Watts' group at the University of Oxford. The main part of the project is thus related to the student's ongoing PhD project in ...
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
|Data from Web of Science|
(References retrieved automatically from State of São Paulo Research Institutions)
MENDES, Luis Felipe Santos. Caracterização estrutural e dinâmica da proteína de estruturação e compactação do complexo de Golgi (GRASP) em solução. 2018. Tese (Doutorado) – Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto. Universidade de São Paulo (USP). Ribeirão Preto.