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Richard John Ward

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Universidade de São Paulo (USP). Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP)  (Institutional affiliation for the last research proposal)
Birthplace: Inglaterra

Graduate of Physiology from the University of Sheffield, UK (1983) and PhD in Biophysics from The Oxford Research Unit Oxford (1987). Dr. Ward as a wide experience in Biophysics with emphasis on the structure/function relationships of proteins, with expertise in the following subjects: directed evolution of proteins, protein engineering of industrial enzymes, nanotechnology and biomimetic systems. (Source: Lattes Curriculum)

Research grants
Scholarships in Brazil
Scholarships abroad
FAPESP support in numbers * Updated December 07, 2019
Total / Available in English
Most frequent collaborators in research granted by FAPESP
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Use this Research Supported by FAPESP (BV/FAPESP) channel only to send messages referring to FAPESP-funded scientific projects.


 

 

 

 

Scientific publications resulting from Research Grants and Scholarships under the grantee's responsibility (42)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

Publications33
Citations304
Cit./Article9.2
Data from Web of Science

ALPONTI, JULIANA SANCHEZ; MALDONADO, RAQUEL FONSECA; WARD, RICHARD J.. Thermostabilization of Bacillus subtilis GH11 xylanase by surface charge engineering. International Journal of Biological Macromolecules, v. 87, p. 522-528, . Web of Science Citations: 11.

RIBEIRO, LUCAS FERREIRA; TULLMAN, JENNIFER; NICHOLES, NATHAN; BERGAMACHI SILVA, SERGIO RUSCHI; VIEIRA, DAVI SERRADELLA; OSTERMEIER, MARC; WARD, RICHARD JOHN. A xylose-stimulated xylanase-xylose binding protein chimera created by random nonhomologous recombination. BIOTECHNOLOGY FOR BIOFUELS, v. 9, . Web of Science Citations: 13.

RIBEIRO, LUCAS F.; BRESSAN, FABIANA; FURTADO, GILVAN P.; MEIRELES, FLAVIO; WARD, RICHARD J.. D-Xylose detection in Escherichia coli by a xylose binding protein-dependent response. Journal of Biotechnology, v. 168, n. 4, p. 440-445, . Web of Science Citations: 3.

FONSECA, RAQUEL GOMES; FERREIRA, TATIANA LOPES; WARD, RICHARD J.. Refolding and purification of the human secreted group IID phospholipase A(2) expressed as inclusion bodies in Escherichia coli. Protein Expression and Purification, v. 67, n. 2, p. 82-87, . Web of Science Citations: 5.

FERREIRA, TATIANA LOPES; WARD, RICHARD J.. The interaction of bothropstoxin-I (Lys49-PLA(2)) with liposome membranes. Toxicon, v. 54, n. 4, p. 525-530, . Web of Science Citations: 2.

FONSECA-MALDONADO, RAQUEL; FERREIRA, TATIANA LOPES; WARD, RICHARD J.. The bactericidal effect of human secreted group IID phospholipase A(2) results from both hydrolytic and non-hydrolytic activities. Biochimie, v. 94, n. 6, p. 1437-1440, . Web of Science Citations: 3.

FURTADO, GILVAN PESSOA; RIBEIRO, LUCAS FERREIRA; SANTOS, CAMILA RAMOS; TONOLI, CELISA CALDANA; DE SOUZA, ANGELICA RODRIGUES; OLIVEIRA, RENATA ROCHA; MURAKAMI, MARIO TYAGO; WARD, RICHARD JOHN. Biochemical and structural characterization of a beta-1,3-1,4-glucanase from Bacillus subtilis 168. Process Biochemistry, v. 46, n. 5, p. 1202-1206, . Web of Science Citations: 29.

RULLER, ROBERTO; ALPONTI, JULIANA; DELIBERTO, LAILA APARECIDA; ZANPHORLIN, LETICIA MARIA; MACHADO, CARLA BOTELHO; WARD, RICHARD JOHN. Concommitant adaptation of a GH11 xylanase by directed evolution to create an alkali-tolerant/thermophilic enzyme. PROTEIN ENGINEERING DESIGN & SELECTION, v. 27, n. 8, p. 255-262, . Web of Science Citations: 12.

MACHADO, CARLA BOTELHO; CITADINI, ANA PAULA; GOLDBECK, ROSANA; DE LIMA, EVANDRO ANTONIO; FIGUEIREDO, FERNANDA LOPES; DA SILVA, TONY MARCIO; HOFFMAM, ZAIRA BRUNA; DE SOUSA, AMANDA SILVA; SQUINA, FABIO MARCIO; TEIXEIRA DE MORAES POLIZELI, MARIA DE LOURDES; et al. Increased biomass saccharification by supplementation of a commercial enzyme cocktail with endo-arabinanase from Bacillus licheniformis. Biotechnology Letters, v. 37, n. 7, p. 1455-1462, . Web of Science Citations: 3.

ARAGAO, ELISANGELA APARECIDA; VIEIRA, DAVI SERRADELLA; CHIOATO, LUCIMARA; FERREIRA, TATIANA LOPES; LOURENZONI, MARCOS ROBERTO; SILVA, SAMUEL REGHIM; WARD, RICHARD JOHN. Characterization of suramin binding sites on the human group IIA secreted phospholipase A(2) by site-directed mutagenesis and molecular dynamics simulation. Archives of Biochemistry and Biophysics, v. 519, n. 1, p. 17-22, . Web of Science Citations: 1.

ARAGAO, E. A.; CHIOATO, L.; FERREIRA, T. L.; MEDEIROS, A. I. DE; SECATTO, A.; FACCIOLI, L. H.; WARD, R. J.. Suramin inhibits macrophage activation by human group IIA phospholipase A(2), but does not affect bactericidal activity of the enzyme. Inflammation Research, v. 58, n. 4, p. 210-217, . Web of Science Citations: 3.

BORTOLETO-BUGS‚ R.K.; BUGS‚ M.R.; NETO‚ A.A.; WARD‚ RJ. A micelle nucleation model for the interaction of dodecyl sulphate with Lys49-phospholipases A2. Biophysical Chemistry, v. 125, n. 1, p. 213-220, .

GUAZZARONI, MARIA-EUGENIA; SILVA-ROCHA, RAFAEL; WARD, RICHARD JOHN. Synthetic biology approaches to improve biocatalyst identification in metagenomic library screening. MICROBIAL BIOTECHNOLOGY, v. 8, n. 1, p. 52-64, . Web of Science Citations: 25.

FONSECA-MALDONADO, RAQUEL; RIBEIRO, LUCAS F.; FURTADO, GILVAN P.; ARRUDA, LETICIA M.; MELEIRO, LUANA P.; ALPONTI, JULIANA S.; BOTELHO-MACHADO, CARLA; VIEIRA, DAVI S.; BONNEIL, ERIC; MELO FURRIEL, ROSA DOS PRAZERES; et al. Synergistic action of co-expressed xylanase/laccase mixtures against milled sugar cane bagasse. Process Biochemistry, v. 49, n. 7, p. 1152-1161, . Web of Science Citations: 9.

CHIOATO‚ L.; DE OLIVEIRA‚ A.H.C.; RULLER‚ R.; SÁ‚ J.M.; WARD‚ R.J.. Distinct sites for myotoxic and membrane-damaging activities in the C-terminal region of a Lys49-phospholipase A2.. Biochemical Journal, v. 366, n. Pt 3, p. 971, .

SÁ‚ J.M.; CHIOATO‚ L.; FERREIRA‚ T.L.; DE OLIVEIRA‚ A.H.C.; RULLER‚ R.; ROSA‚ J.C.; GREENE‚ L.J.; WARD‚ R.J.. Topology of the substrate-binding site of a Lys49-phospholipase A2 influences Ca2+-independent membrane-damaging activity. Biochemical Journal, v. 382, n. Pt 1, p. 191, .

MURAKAMI‚ M.T.; ARNI‚ R.K.; VIEIRA‚ D.S.; DEGRÈVE‚ L.; RULLER‚ R.; WARD‚ R.J.. Correlation of temperature induced conformation change with optimum catalytic activity in the recombinant G/11 xylanase A from Bacillus subtilis strain 168 (1A1). FEBS Letters, v. 579, n. 28, p. 6505-6510, .

DEGRÈVE‚ L.; CARLOS BORIN‚ A.; MAZZÉ‚ F.M.; RODRIGUES‚ A.L.G.. Molecular simulation of a phase separation in a non-primitive electrolyte solution. Chemical Physics, v. 265, n. 2, p. 193-205, .

FERREIRA, TATIANA LOPES; RULLER, ROBERTO; CHIOATO, LUCIMARA; WARD, RICHARD J.. Insights on calcium-independent phospholipid membrane damage by Lys49-PLA(2) using tryptophan scanning mutagenesis of bothropstoxin-I from Bothrops jararacussu. Biochimie, v. 90, n. 9, p. 1397-1406, . Web of Science Citations: 11.

TULLMAN, JENNIFER; NICHOLES, NATHAN; DUMONT, MATT R.; RIBEIRO, LUCAS F.; OSTERMEIER, MARC. Enzymatic protein switches built from paralogous input domains. Biotechnology and Bioengineering, v. 113, n. 4, p. 852-858, . Web of Science Citations: 11.

FONSECA, RAQUEL GOMES; FERREIRA, TATIANA LOPES; WARD, RICHARD J.. Refolding and purification of the human secreted group IID phospholipase A(2) expressed as inclusion bodies in Escherichia coli. Protein Expression and Purification, v. 67, n. 2, p. 82-87, . Web of Science Citations: 5.

FERREIRA, TATIANA LOPES; WARD, RICHARD J.. The interaction of bothropstoxin-I (Lys49-PLA(2)) with liposome membranes. Toxicon, v. 54, n. 4, p. 525-530, . Web of Science Citations: 2.

ARAGAO, E. A.; CHIOATO, L.; FERREIRA, T. L.; MEDEIROS, A. I. DE; SECATTO, A.; FACCIOLI, L. H.; WARD, R. J.. Suramin inhibits macrophage activation by human group IIA phospholipase A(2), but does not affect bactericidal activity of the enzyme. Inflammation Research, v. 58, n. 4, p. 210-217, . Web of Science Citations: 3.

BORTOLETO-BUGS‚ R.K.; BUGS‚ M.R.; NETO‚ A.A.; WARD‚ RJ. A micelle nucleation model for the interaction of dodecyl sulphate with Lys49-phospholipases A2. Biophysical Chemistry, v. 125, n. 1, p. 213-220, .

RIBEIRO, LUCAS FERREIRA; NICHOLES, NATHAN; TULLMAN, JENNIFER; COSTA RIBEIRO, LILIANE FRAGA; FUZO, CARLOS ALESSANDRO; VIEIRA, DAVI SERRADELLA; FURTADO, GILVAN PESSOA; OSTERMEIER, MARC; WARD, RICHARD JOHN. Insertion of a xylanase in xylose binding protein results in a xylose-stimulated xylanase. BIOTECHNOLOGY FOR BIOFUELS, v. 8, . Web of Science Citations: 14.

ALVES, LUANA DE FATIMA; MELEIRO, LUANA PARRAS; SILVA, ROBERTO N.; WESTMANN, CAUA ANTUNES; GUAZZARONI, MARIA-EUGENIA. Novel Ethanol- and 5-Hydroxymethyl Furfural-Stimulated beta-Glucosidase Retrieved From a Brazilian Secondary Atlantic Forest Soil Metagenome. FRONTIERS IN MICROBIOLOGY, v. 9, . Web of Science Citations: 1.

DE CARVALHO, DANIELLA ROMANO; CARLI, SIBELI; MELEIRO, LUANA PARRAS; ROSA, JOSE CESAR; CAVALCANTE DE OLIVEIRA, ARTHUR HENRIQUE; JORGE, JOAO ATILIO; MELO FURRIEL, ROSA PRAZERES. A halotolerant bifunctional beta-xylosidase/alpha-L-arabinofuranosidase from Colletotrichum graminicola: Purification and biochemical characterization. International Journal of Biological Macromolecules, v. 114, p. 741-750, . Web of Science Citations: 4.

SANTOS SALGADO, JOSE CARLOS; MELEIRO, LUANA PARRAS; CARLI, SIBELI; WARD, RICHARD JOHN. Glucose tolerant and glucose stimulated beta-glucosidases - A review. Bioresource Technology, v. 267, p. 704-713, . Web of Science Citations: 14.

CARLI, SIBELI; MELEIRO, LUANA PARRAS; WARD, RICHARD JOHN. Biochemical and kinetic characterization of the recombinant GH28 Stereum purpureum endopolygalacturonase and its biotechnological application. International Journal of Biological Macromolecules, v. 137, p. 469-474, . Web of Science Citations: 0.

WARD‚ R.J.; CHIOATO‚ L.; DE OLIVEIRA‚ A.H.C.; RULLER‚ R.; SÁ‚ J.M.. Active-site mutagenesis of a Lys49-phospholipase A2: biological and membrane-disrupting activities in the absence of catalysis.. Biochemical Journal, v. 362, n. Pt 1, p. 89, .

RULLER‚ R.; ARAGAO‚ EA; CHIOATO‚ L.; FERREIRA‚ T.L.; DE OLIVEIRA‚ AHC; SA‚ JM; WARD‚ RJ. A predominant role for hydrogen bonding in the stability of the homodimer of bothropstoxin-I‚ A lysine 49-phospholipase A2. Biochimie, v. 87, n. 11, p. 993-1003, .

APARECIDA ARAGÃO‚ E.; CHIOATO‚ L.; WARD‚ R.J.. Permeabilization of E. coli K12 inner and outer membranes by bothropstoxin-I‚ A LYS49 phospholipase A2 from Bothrops jararacussu. Toxicon, v. 51, n. 4, p. 538-546, .

SILVA, SERGIO BERGAMACHI; PINHEIRO, MATHEUS PINTO; FUZO, CARLOS ALESSANDRO; SILVA, SAMUEL REGHIM; FERREIRA, TATIANE LOPES; LOURENZONI, MARCOS ROBERTO; NONATO, M. CRISTINA; VIEIRA, DAVI SERRADELLA; WARD, RICHARD JOHN. The role of local residue environmental changes in thermostable mutants of the GH11 xylanase from Bacillus subtilis. International Journal of Biological Macromolecules, v. 97, p. 574-584, . Web of Science Citations: 2.

MELEIRO, LUANA PARRAS; SANTOS SALGADO, JOSE CARLOS; MALDONADO, RAQUEL FONSECA; CARLI, SIBELI; BERALDO MORAES, LUIZ ALBERTO; WARD, RICHARD JOHN; JORGE, JOAO ATILIO; MELO FURRIEL, ROSA PRAZERES. Engineering the GH1 beta-glucosidase from Humicola insolens: Insights on the stimulation of activity by glucose and xylose. PLoS One, v. 12, n. 11, . Web of Science Citations: 9.

CARNEIRO, LARA A. B. C.; WARD, RICHARD J.. Functionalization of paramagnetic nanoparticles for protein immobilization and purification. Analytical Biochemistry, v. 540, p. 45-51, . Web of Science Citations: 4.

RIBEIRO, LILIANE F. C.; DE LUCAS, ROSYMAR C.; VITCOSQUE, GABRIELA L.; RIBEIRO, LUCAS F.; WARD, RICHARD J.; RUBIO, MARCELO V.; DAMASIO, ANDRE R. L.; SQUINA, FABIO M.; GREGORY, REBECCA C.; WALTON, PAUL H.; et al. A novel thermostable xylanase GH10 from Malbranchea pulchella expressed in Aspergillus nidulans with potential applications in biotechnology. BIOTECHNOLOGY FOR BIOFUELS, v. 7, . Web of Science Citations: 30.

FONSECA-MALDONADO, RAQUEL; VIEIRA, DAVI SERRADELLA; ALPONTI, JULIANA SANCHEZ; BONNEIL, ERIC; THIBAULT, PIERRE; WARD, RICHARD JOHN. Engineering the Pattern of Protein Glycosylation Modulates the Thermostability of a GH11 Xylanase. Journal of Biological Chemistry, v. 288, n. 35, p. 25522-25534, . Web of Science Citations: 33.

FURTADO, G. P.; RIBEIRO, L. F.; LOURENZONI, M. R.; WARD, R. J.. A designed bifunctional laccase/beta-1,3-1,4-glucanase enzyme shows synergistic sugar release from milled sugarcane bagasse. PROTEIN ENGINEERING DESIGN & SELECTION, v. 26, n. 1, p. 15-23, . Web of Science Citations: 20.

FURTADO, GILVAN P.; SANTOS, CAMILA R.; CORDEIRO, ROSA L.; RIBEIRO, LUCAS F.; DE MORAES, LUIZ A. B.; DAMASIO, ANDR R. L.; POLIZELI, MARIA DE LOURDES T. M.; LOURENZONI, MARCOS R.; MURAKAMI, MARIO T.; WARD, RICHARD J.. Enhanced xyloglucan-specific endo-beta-1,4-glucanase efficiency in an engineered CBM44-XegA chimera. Applied Microbiology and Biotechnology, v. 99, n. 12, p. 5095-5107, . Web of Science Citations: 10.

VIEIRA, DAVI SERRADELLA; WARD, RICHARD JOHN. Conformation analysis of a surface loop that controls active site access in the GH11 xylanase A from Bacillus subtilis. Journal of Molecular Modeling, v. 18, n. 4, p. 1473-1479, . Web of Science Citations: 9.

CHIOATO‚ L.; WARD‚ R.J.. Mapping structural determinants of biological activities in snake venom phospholipases A2 by sequence analysis and site directed mutagenesis. Toxicon, v. 42, n. 8, p. 869-883, .

CHIOATO, LUCIMARA; ARAGAO, ELISANGELA APARECIDA; FERREIRA, TATIANA LOPES; WARD, RICHARD J.. Active site mutants of human secreted Group IIA Phospholipase A(2) lacking hydrolytic activity retain their bactericidal effect. Biochimie, v. 94, n. 1, p. 132-136, . Web of Science Citations: 4.

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

ALVES, Luana de Fátima. Criação de uma enzima multifuncional feruloil esterase/acetil-xilano esterase por desenho racional. 2016. Dissertação (Mestrado) - Faculdade de Medicina de Ribeirão Preto. Universidade de São Paulo (USP). Ribeirão Preto.

ALVES, Adriana Rodrigues. Investigação da relação entre estrutura e função em uma fospolipase PLA2 utilizando mutagênese sítio-dirigida. . 101f. Dissertação (Mestrado) - Instituto de Biociências, Letras e Ciências Exatas. Universidade Estadual Paulista. São José do Rio Preto.

ARAGÃO, Elisângela Aparecida. Efeito da suramina na atividade da fosfolipase A2 secretada humana do grupo IIA. 2008. Tese (Doutorado) – Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto. Universidade de São Paulo (USP). Ribeirão Preto.

MOLINA, Gustavo Avelar. Caracterização biofísica da dinâmica catalítica de uma xilanase GH11. 2016. Dissertação (Mestrado) - Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto. Universidade de São Paulo (USP). Ribeirão Preto.

ARAGÃO, Elisângela Aparecida. Efeito bactericida de Fosfolipases A2-Lys49: o papel da região C-terminal na atividade de Bothropstoxina-I em membranas biológicas e artificiais.. 2005. Dissertação (Mestrado) - Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto. Universidade de São Paulo (USP). Ribeirão Preto.

BUGS, Raquel Kely Bortoleto. Transformações estruturais e função de fosfolipase. . 115f. Tese (Doutorado) – . Universidade Estadual Paulista "Júlio de Mesquita Filho" Instituto de Biociências, Letras e Ciências Exatas. São José do Rio Preto.

Patent applications

PARTÍCULAS FERROMAGNÉTICAS E SEUS USOS BR1020160210950 - Universidade de São Paulo (USP). Richard John Ward; Lara Aparecida Buffoni de Campos Carneiro - September 2016, 13

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