Mario Henrique de Barros
CV Lattes
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Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB) (Institutional affiliation for the last research proposal) Birthplace: Brazil
Master and PhD studies in Genetics at IB/USP. Former pos-doc of Columbia University (NY-USA) and Biochemistry Department -IQ/USP. Nowadays is an Associate Professor at the Department of Microbiology - ICB/USP, using Saccharomyces cerevisiae as model for studies related to mitochondria bioenergetics and biogenesis. (Source: Lattes Curriculum)
- Study of mitochondrial translation factors, AP.R
Abstract
Our laboratory studies the mitochondrial biogenesis in Saccharomyces cerevisiae. In the last years we have characterized new factors involved in mitochondrial translation such as bL34, Msc6p and Aep3p. Here our first aim is to discriminate Msc6p function, with the identification of genetic interactors, proteins that interact with Msc6p as well as to identify the putative associated RNAs...
- Co-regulatory studies onto mitochondrial ribosome biogenesis and cytochrome oxidase assembly, AV.EXT
Abstract
The assembly of the mitochondrial respiratory complexes depends on proteins encoded by two different genomes, an unique situation in human cells that arise our interest on regulatories mechanisms involved in the proper steichiometric assembly of the respiratory complexes. Here, we propose to study a possible regulatory interconnection between cytochrome c oxidase (COX) assembly and the ...
- Saccharomyces cerevisiae as a model for mitochondrial translation studies, AP.R
Abstract
Our aim is to investigate mitochondria biogenesis through the study of genes with unknown function. In the last ten years we have characterized the following genes: COX23, COX24, COQ9, COQ10, ATP25, GTF1 and MTG3. They are involved in different aspects of mitochondria biogenesis such as the assembly of cytochrome c oxidase, CoQ function, ATP synthase formation, and mitochondria translat...
- Study of mitochondrial proteins of unknown function and their respective effects on cell viability, AP.R
Abstract
The present project aims the characterization of Saccharomyces cerevisiae ORFs still identified with unknown function. Cells depleted for most of these ORFs do not show any clear phenotype, making the study of their respective function more problematic. However, our previous works in this subject were very profitable opening new interesting projects, such as those involved in coenzyme...
(Only some records are available in English at this moment)
- ATCO1, a mitochondrial master regulator of ATP synthase and cytochrome oxidase biogenesis?, BP.PD
Abstract
Fungal and mammalian ATP Synthase and Cytochrome C oxidase (COX) are examples of mitochondrial hetero-oligomeric complexes whose assembly depends on the expression of mitochondrial and nuclear genes. In prior studies, yeast ATP synthase was shown to be assembled from three modules, each formed by a separate pathway. The simplest module is a ring, which in yeast consists of 10 identical ...
- Study of the formation of the mitoribosome exit tunnel and impact in mitochondria translation, BP.IC
Abstract
Our lab aim is the study of mitochondria biogenesis in yeast. Here we propose to study the formation of the mitoribosome exit tunnel and the impact of protein extensions of ribosome proteins not present in other types of ribosomes, and therefore unique of the mitoribosome. After the generation of new alleles we will test them in the respective null mutants checking for respiratory and t...
- Search for the gene coding for the amidotransferase connecting subunit in humans and the study of the function of MSC6 in yeast, BP.IC
Abstract
mRNA translation requires the correct association of the tRNA with the cognate aminoacid. This process is carried by a specific aminoacyl-tRNA synthase. Some bacteria and organelles do not have the complete of aminoacyl tRNA synthases and extra transamidation reactions are therefore required for the aminoacid inespecifically associated to the tRNA. For instance this process occurs in th...
- Study of the regulation of alternative polyadenylation of precursors of messenger RNA by the energetic metabolism and aging in Saccharomyces cerevisiae, BP.PD
Abstract
In eukaryotes, RNA polymerase II is responsible for the transcription of the precursors of messenger RNA, which undergo an extensive co-transcriptional processing whose final result is their maturation. Except for the transcripts of histone genes, the precursors of every other messenger RNAs present endonucleolytic cleavage and polyadenylation at their 3' ends. Notably, the cleavage/ po...
(Only some records are available in English at this moment)
- Study on mitochondria-nucleus regulatory and communication ways in controlling co-expression of Saccharomyces cerevisiae respiratory complexes, BE.PQ
Abstract
Mitochondria and nucleus crosstalk is continuous and regulated by a number of gene products and secondary metabolites. External signals are also detected by different pathways, leading to a differential expression of genes involved in the respiratory electron chain. Mitochondrial dysfunction and metabolites generated by its Redox state are also detected by nucleus receptors, resulting i...
| 1 / 1 | Ongoing research grants |
| 7 / 5 | Completed research grants |
| 1 / 1 | Ongoing scholarships in Brazil |
| 18 / 7 | Completed scholarships in Brazil |
| 2 / 1 | Completed scholarships abroad |
| 29 / 15 | All research grants and scholarships |
| Associated processes | |
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
| Publications | 12 |
| Citations | 69 |
| Cit./Article | 5.8 |
| Data from Web of Science | |
GOMES, FERNANDO; TAHARA, ERICH B.; BUSSO, CLEVERSON; KOWALTOWSKI, ALICIA J.; BARROS, MARIO H.. nde1 deletion improves mitochondrial DNA maintenance in Saccharomyces cerevisiae coenzyme Q mutants. Biochemical Journal, v. 449, n. 3, p. 595-603, FEB 1 2013. Web of Science Citations: 13. (11/07366-5)
BUSSO, CLEVERSON; FERREIRA-JUNIOR, JOSE RIBAMAR; PAULELA, JANAINA A.; BLEICHER, LUCAS; DEMASI, MARILENE; BARROS, MARIO H.. Coq7p relevant residues for protein activity and stability. Biochimie, v. 119, p. 92-102, DEC 2015. Web of Science Citations: 8. (13/07937-8, 11/07366-5)
BARROS, MARIO H.; TZAGOLOFF, ALEXANDER. Aep3p-dependent translation of yeast mitochondrial ATP8. MOLECULAR BIOLOGY OF THE CELL, v. 28, n. 11, p. 1426-1434, JUN 1 2017. Web of Science Citations: 4. (13/09482-8, 13/07937-8)
ARITA, JULIANA HARUMI; BARROS, MARIO H.; RAVAGNANI, FELIPE GUSTAVO; ZIOSI, MARCELLO; SANCHES, LIVIA RENTAS; PICOSSE, FABIOLA ROSA; LOPES, TANIA OLIVEIRA; AGUIAR, PATRICIA DE CARVALHO; MACABELLI, CAROLINA HABERMANN; CHIARATTI, MARCOS R.; et al. Metabolic studies of a patient harbouring a novel S487L mutation in the catalytic subunit of AMPK. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE, v. 1864, n. 5, A, p. 1896-1903, MAY 2018. Web of Science Citations: 0. (15/23549-3, 13/07937-8, 10/51924-0, 11/07366-5)
MODA, BRUNO S.; FERREIRA-JUNIOR, JOSE RIBAMAR; BARROS, MARIO H.. Partial suppression of the respiratory defect of qrs1/her2 glutamyl-tRNA amidotransferase mutants by overexpression of the mitochondrial pentatricopeptide Msc6p. CURRENT GENETICS, v. 62, n. 3, p. 607-617, AUG 2016. Web of Science Citations: 6. (13/09482-8)
FERREIRA-JUNIOR, JOSE RIBAMAR; BLEICHER, LUCAS; BARROS, MARIO H.. Her2p molecular modeling, mutant analysis and intramitochondrial localization. Fungal Genetics and Biology, v. 60, n. SI, p. 133-139, NOV 2013. Web of Science Citations: 1. (11/07366-5)
GUEDES-MONTEIRO, RAQUEL FONSECA; FERREIRA-JUNIOR, JOSE RIBAMAR; BLEICHER, LUCAS; NOBREGA, FRANCISCO G.; BARRIENTOS, ANTONI; BARROS, MARIO H.. Mitochondrial ribosome bL34 mutants present diminished translation of cytochrome c oxidase subunits. Cell Biology International, v. 42, n. 6, SI, p. 630-642, JUN 2018. Web of Science Citations: 3. (13/09482-8, 13/07937-8, 11/18892-0)
ZAMPOL, MARIANA A.; BARROS, MARIO H.. Melatonin improves survival and respiratory activity of yeast cells challenged by alpha-synuclein and menadione. YEAST, v. 35, n. 3, p. 281-290, MAR 2018. Web of Science Citations: 3. (11/03583-1, 11/07366-5)
ZAMPOL, MARIANA A.; BUSSO, CLEVERSON; GOMES, FERNANDO; FERREIRA-JUNIOR, JOSE RIBAMAR; TZAGOLOFF, ALEXANDER; BARROS, MARIO H.. Over-expression of COQ10 in Saccharomyces cerevisiae inhibits mitochondrial respiration. Biochemical and Biophysical Research Communications, v. 402, n. 1, p. 82-87, NOV 5 2010. Web of Science Citations: 16. (07/01092-5)
BUSSO, CLEVERSON; TAHARA, ERICH B.; OGUSUCU, RENATA; AUGUSTO, OHARA; FERREIRA-JUNIOR, JOSE RIBAMAR; TZAGOLOFF, ALEXANDER; KOWALTOWSKI, ALICIA J.; BARROS, MARIO H.. Saccharomyces cerevisiae coq10 null mutants are responsive to antimycin A. FEBS Journal, v. 277, n. 21, p. 4530-4538, NOV 2010. Web of Science Citations: 14. (07/01092-5)
RIBEIRO FRANCO, LETICIA VELOSO; MODA, BRUNO S.; SOARES, MARIA A. K. M.; BARROS, MARIO H.. Msc6p is required for mitochondrial translation initiation in the absence of formylated Met-tRNA(fMet). FEBS Journal, v. 286, n. 7, p. 1407-1419, APR 2019. Web of Science Citations: 1. (13/09482-8, 13/07937-8, 17/23921-5, 16/25907-7)
GUEDES-MONTEIRO, RAQUEL F.; FRANCO, LETICIA V. R.; MODA, BRUNO S.; TZAGOLOFF, ALEXANDER; BARROS, MARIO H.. 5 ` processing of Saccharomyces cerevisiae mitochondrial tRNAs requires expression of multiple genes. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, v. 1866, n. 5, p. 806-818, MAY 2019. Web of Science Citations: 0. (13/09482-8, 13/07937-8, 17/23921-5)
(References retrieved automatically from State of São Paulo Research Institutions)
BUSSO, Cleverson. Caracterização funcional da proteína Coq10p de Saccharomyces cerevisiae na atividade respiratória da coenzima Q.. 2010. Tese (Doutorado) – Instituto de Ciências Biomédicas. Universidade de São Paulo (USP). São Paulo. (06/03713-4)
PAULELA, Janaina Areias. Caracterização de linhagens de saccharomyces cerevisiae deficientes na biossíntese da Coenzima Q.. 2018. Tese (Doutorado) – Instituto de Ciências Biomédicas. Universidade de São Paulo (USP). São Paulo. (12/11524-8)
MONTEIRO, Raquel Fonseca Guedes. Estudo de proteínas que afetam a tradução mitocondrial em Saccharomyces cerevisiae.. 2017. Tese (Doutorado) – Instituto de Ciências Biomédicas. Universidade de São Paulo (USP). São Paulo. (11/18892-0)
ZAMPOL, Mariana Alba. Efeito de antioxidantes naturais em modelos parkinsonianos de Saccharomyces cerevisiae.. 2013. Dissertação (Mestrado) - Instituto de Ciências Biomédicas. Universidade de São Paulo (USP). São Paulo. (11/03583-1)