Alexandre Keiji Tashima

• Abstract

  Antivenoms are the only effective treatment for accidents involving venomous snakes, saving countless lives over more than a century. Despite their great value, antivenoms often cause adverse reactions. The formulations contain a substantial fraction of non-specific antibodies and serum proteins that do not neutralize venom toxins. Brazil records a high number of snakebite incidents annua…

  Study of the humoral response and characterization of antibodies in individuals involved in multiple snakebite incidents, AP.R

• Abstract

  The Laboratory of Mass Spectrometry Applied to Biomolecules (LEMBio) is a multi-user center established at EPM/UNIFESP and specialized in analyses based on mass spectrometry. Since its inception, LEMBio has served a broad range of researchers and their research projects. However, the currently used mass spectrometers have been in place for over 10 years, and during this period, there has …

  EMU: Acquisition of a high-resolution mass spectrometer with an extended mass range for the analysis of antibodies, biopharmaceuticals, peptides, and metabolites., AP.INFRA7.PM

• Abstract

  Humoral and cellular responses against COVID-19 are key to suppressing viral replication, preventing severe disease symptoms and possible reinfections. Understanding how lymphocytes respond to viral infection and vaccination can give support to possible strategies to combat the disease. The cellular environment is complex and dynamic, in addition to being organized by subcellular compartm…

  Studies of Spatial Proteomics and Transcriptomics Applied to Cell Systems., AV.EXT

• Abstract

  The SARS-CoV-2, which causes COVID-19, spread rapidly to all regions of the world, causing the global pandemic in March 2020. The preventive method against the viral infection currently adopted by several countries is vaccination. A large number of clinical studies are underway with the aim of finding effective drugs or procedures against the disease. One option is the immunotherapy with …

  Anti-SARS-CoV-2 antibodies in the plasma of vaccinees: dynamics of humoral response, sequencing, expression and effectiveness of recombinants for therapy and diagnosis, AP.R

• Abstract

  Venomous animals are amongst the most successful animals on Earth's evolutionary history. One of the important factors for their success is the production of venoms, used to subdue prey and as protection against possible predators. Most active toxins are proteins and peptides, elaborated and refined over millions of years of evolution. Venom toxins have served as leads for molecules with …

  Peptidomics of Brazilian snake and spider venoms, AP.R

(Only some records are available in English at this moment)

• Abstract

  Antivenom is the only effective treatment for snakebite accidents, saving countless lives for over a century. Despite its great value, antivenoms frequently cause adverse reactions. Brazil registers a high number of snakebite accidents annually. Due to the efficiency of the national distribution of antivenom, only a small fraction of these cases result in death. This means that a large nu…

  Sequencing of antibodies against snake venom toxins by mass spectrometry, BP.IC

• Abstract

  In 2020, the world faced the SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2), which was responsible for millions of deaths and patients with sequelae from COVID-19 (coronavirus disease 2019). Since then, science has worked intensively to discover preventive solutions against the disease. A few months after the pandemic was declared, the first vaccine against the disease was p…

  Sequencing of a SARS-CoV-2 anti-spike S1 monoclonal antibody using mass spectrometry., BP.IC

• Abstract

  O bolsista TT-1 aprenderá e contribuirá com o desenvolvimento de ferramentas de bioinformática direcionadas à análise de dados de sequenciamento de RNA, obtidas principalmente pela plataforma MinION Mk1C (Oxford Nanopore Technologies). O bolsista contribuirá com a instalação de ferramentas no ambiente Unix/Linux, voltadas à análise de sequências, e elaborará rotinas para a clusterização e…

  Elaboration of routines and preparation of RNA-Seq data for clustering and alignment analysis, BP.TT

• Abstract

  A bolsista TT-3 aprenderá e contribuirá com o desenvolvimento de protocolos de preparação de cDNA e amplicons de sequências de cadeias leves e pesadas de anticorpos contra o SARS-CoV-2. Além disso, a bolsista realizará protocolos de purificação de anticorpos do plasma e aprenderá os protocolos de análise proteômica por espectrometria de massas. Participará da elaboração de ferramentas de …

  Analysis of antibody sequences by RNA-Seq and mass spectrometry., BP.TT

(Only some records are available in English at this moment)