Amando Siuiti Ito

• Abstract

  The fluid mosaic model proposed that the lipid structuration as an essential for the biological activity of proteins immersed in the cell membrane. The model was improved taking into account the formation of specialized lipid domains as important structural factor for the membrane activity. Kusumi and collaborators proposed that the membrane is hierarchically organized in dynamic structur…

  Single molecule fluorescence in biological membranes, AV.EXT

• Abstract

  Many biological processes are related to interactions between molecules and complex structures like the cell membrane, where organized lipid domains play relevant role. Even interactions in two components systems, like enzyme and substrate, depend on complex conformational dynamics, where heterogeneities are essential for the biological function. Advances in studies of both systems were a…

  Heterogeneities in lipids and proteins structures: studies by advanced fluorescence techniques, AP.R

• Abstract

  This proposal is aimed at the acquisition of equipment needed in order to conduct experiments involving fluorescence correlation spectroscopy (FCS), which is a technique for the analysis of biomolecules at extremely low concentrations, with high spatial and temporal resolution. Spontaneous fluctuations in intensity, caused by small deviations in the thermal balance of the system, constitu…

  Acquisition of a fluorescence correlation spectroscope for the measurement of fluorescence correlation and image capture by fluorescence lifetime, AP.EMU

(Only some records are available in English at this moment)

• Abstract

  With FCS (powerful technique for quantitative research on properties of membranes) will be investigated specific conditions of changes in lipid bilayers associated with variations in composition, concentration and temperature controlled and inclusion of various types of molecules,within the bilayer and in its interface with the aqueous medium. Correlating the project with interest in anti…

  Fluorescence correlation spectroscopy in studies of lateral diffusion in model membranes., BP.IC
• Structural properties of model membranes on interaction with anti-Leishmania drugs, BP.DR