CV Lattes
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Pontifícia Universidade Católica de São Paulo (PUC-SP). Faculdade de Ciências Médicas e da Saúde (FCMS) (Institutional affiliation from the last research proposal) Birthplace: Brazil
graduation at Ciências Biológicas from Universidade Federal de São Carlos (2014) and doctorate at Genetics from Universidade Federal de São Carlos (2019). Has experience in Genetics, focusing on Molecular Genetics and of Microorganisms, acting on the following subjects: far-red bioluminescence, p.hirtus, red- glowing railroadworm p.hirtus, phrixotrix luciferase and bioimaging. (Source: Lattes Curriculum)
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The bioluminescent reporter genes that encode luciferases are among the most sensitive and frequently used to monitor non-invasively cellular processes, in real time. Unlike fluorescent reporter genes such as GFP, which have disadvantages such as autoabsorption of irradiated light, autofluorescence and phototoxicity, bioluminescent reporter genes have the advantage of producing their own …
Luciferases are enzymes responsible for bioluminescence. These enzymes catalyze the exergonic highly oxidation of substrates known as luciferins, that the energy is released as light with high efficiency. The firefly luciferase are the most known and used in the biotechnology field as ATP bioindicators reagents, biosensors, cell biomarkers, reporter genes, among other applications. In the…
The firefly luciferin-luciferase system has been used in an enormous variety of bioanalytical applications. Firefly luciferase genes have been widely used as reporters in gene expression studies and for cell tracking in biological and pathological processes, including cell proliferation studies, cytotoxicity assays, and metastasis in model animals, emerging as a novel technology replacing…
Beetle luciferases are responsible for the production of different bioluminescence colors from green to red using the same firefly D-luciferin. Some luciferin analogues such as 6´amino-analogues can extend the range of bioluminescence colors. Among several beetle luciferases that have cloned and been studied, Phrixothrix hirtus (PxRE) railroadworm luciferase is still the only one that nat…
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