José Franco da Silveira Filho

• Abstract

  Infection by Trypanosoma cruzi, the etiological agent of Chagas Disease remains a major public health problem in Latin America, home of most people infected. In nonendemic regions like/such as the US, Europe, Japan, and Australia there is growing concern about the risks of transmission by blood transfusion (and organ transplant). Considering that T. cruzi taxon comprises genetically disti…

  Trypanosoma cruzi: intra and interspecific genomic variability and mechanisms of cell invasion/egress, AP.TEM

• Abstract

  Trypanosoma cruzi is the causative agent of Chagas' disease that affects 15-16 million people in America. Vectorial transmission of T. cruzi still occurs in several regions. Migration from endemic areas to developed countries has led to new cases in the US, Europe, Japan and Australia. Since Triatoma infestans has been controlled and transmission by blood transfusion, halted, oral infecti…

  Molecular and cellular biology of the parasitism by Trypanosoma cruzi, AP.TEM

• Abstract

  Trypanosoma cruzi, the etiological agent of Chagas' disease, is a highly relevant microorganism from both medical and biological points of view. Although vectorial transmission is mostly under control in the Southern Cone, T cruzi transmission still prevails in Latin America where 15-16 million people are infected. Outbreaks of acute cases of Chagas' disease by oral infection have been re…

  Molecular studies on Trypanosoma cruzi and its interaction with cells and factors from the host in vitro and in vivo, AP.TEM

(Only some records are available in English at this moment)

• Abstract

  Trypanosoma cruzi is a parasitic protozoan that shows remarkable genetic variability among strains and clones derived from the same strain, with significant differences in the genotypic and phenotypic traits, such as infectivity and pathogenicity patterns, as well as variations in the molecular karyotype. These changes may play an important role in the adaptive response in different hosts…

  Analysis of chromosome number variation (aneuploidy) in Trypanosoma cruzi by FISH technique ("Fluorescence In Situ Hybridization")., BP.IC

• Abstract

  Trypanosoma cruzi, the etiologic agent of Chagas disease, is a cell highly resistant to ionizing radiation. It can survive doses of gamma irradiation exposure that exceed considerably the absolutely lethal doses for mammalian cells. Lesions more complex and possibly more harmful to the cells are double-stranded breaks in DNA (DSBs), which can be repaired by homologous or nonhomologous rec…

  Ionizing radiation effects and double-stranded breaks (DSBs) on the molecular karyotype of T. cruzi, BP.IC

• Abstract

  Genomic analysis indicates that T. cruzi is diploid for most of its chromosomes. However, aneuploidy events were detected in some chromosomes by aCGH and NGS (next generation sequencing). It has been suggested that aneuploidy may be an important factor in generating genetic diversity in T. cruzi, an organism in which sexual reproduction is absent or very rare. By comparing strains and clo…

  Construction of transgenic lines modified by CRISPR/Cas9 to identify events of aneuploidy in Trypanosoma cruzi, BP.PD

(Only some records are available in English at this moment)