Silvano Mario Attilio Raia

• Abstract

  After the development of modern genetic editing techniques, xenotransplantation became the potential solution to the shortage of organs for transplantation. Since 2017, our group has been leading a research effort for the development of genetically modified pigs for this purpose. With the support of XenoBrasil (startup that has EMS as a partner and some of the leading researchers of this …

  Science for Development Center in Xenotransplantation, AP.CCD

• Abstract

  Motivated by the growing need for transplantation-available organs and the latest developments in genomic editing technologies such as the CRISPR-Cas9 system, significant progress in the efficiency and safety of xenotransplantation techniques has been achieved in the last two years. Genetically engineered pigs developed to prevent receptor hyperacute immune rejection have been successfull…

  Multi-User Equipment approved in grant 18/14275-5: Micromanipulator - equipment for nuclear transfer, AP.EMU

• Abstract

  Motivated by the growing need for transplantation-available organs and the latest developments in genomic editing technologies such as the CRISPR-Cas9 system, significant progress in the efficiency and safety of xenotransplantation techniques has been achieved in the last two years. Genetically engineered pigs developed to prevent receptor hyperacute immune rejection have been successfull…

  Multi-User Equipment approved in grant 18/14275-5: Nucleofector 4D - equipment used for genomic editing, AP.EMU

• Abstract

  The scarcity of organ donations worldwide is considered a major barrier to transplants. In view of this, xenotransplantation has emerged as an important alternative to overcome the shortage of organs needed for transplants. Pigs have rapid maturation, numerous offspring and physiological characteristics similar to humans, which make them ideal candidates to be xenotransplant donors. Howev…

  Development of a multiplex molecular and serological panel for the diagnosis of clinically important pathogens for xenotransplantation, BP.PD
• Production of swine zygotes for generation of genetically modified embryos for xenotransplantation., BP.TT

• Abstract

  The scientific community asserts that the absence of Specific Pathogen-Free (SPF) and Designated Pathogen-Free (DPF) pathogens in xenotransplantation candidate animals is paramount. The real-time polymerase chain reaction (qPCR) is one of the most efficacious methodologies for detecting these pathogens, mainly due to its high sensitivity (1 copy/mL). The selection of samples to be evaluat…

  Standardization of preparation, extraction, and amplification of several types of samples to detect clinically important pathogens for Xenotransplantation realization, BP.IC

(Only some records are available in English at this moment)