Marcelo Damário Gomes

• Abstract

  Muscle wasting or atrophy is a condition associated with major human systemic diseases including, diabetes, cancer, and kidney failure, among others. There is accumulating evidence from comparison of transcriptional profiles that a common set of genes, termed atrogenes, are modulated in atrophying muscles. However, the transcriptional changes that trigger reversion or attenuation of muscl…

  Genes Differentially Expressed During Reversion Skeletal Muscle Atrophy, AP.R

• Abstract

  The FBXO25 mediates degradation of ELK-1 and thus inhibits transcriptional activation of immediate early genes (iEG). Here we show that FBXO25 regulates yet another node of this signaling pathway, by decreasing MAPK/ERK activity. We show that induction of FBXO25 reduced ERK1/2 phosphorylation independently of MEK1/2. Accordingly, in HAP1 FBXO25 knockout cells (FBXO25KO), we observed that …

  FBXO25 Regulates MAPK Signaling Pathway Through Inhibition of ERK1/2 Phosphorylation, PUB.ART

• Abstract

  The dephosphorylation of ERK1/2 is a critical process in regulating the duration of MAPK signaling activation. Recently, we show that FBXO25 interacts, mediates ubiquitination and proteasomal degradation of ELK-1 protooncogene regulator. FBXO25 inhibits the activation of two ELK-1 target genes, c-fos and egr-1, in response to PMA. Downstream ELK-1, FBXO25 mediates the dephosphorylating of…

  High-throughput RNAi library screening to identify novel regulators of ERKs, AP.R

(Only some records are available in English at this moment)

• Abstract

  Phosphatases, respectively, are of great importance for several cellular events. Among the types of protein kinases and protein phosphatases, the mitogen-activated protein kinases and the serine, threonine and tyrosine phosphatases regulating this pathway, stand out for the ability to regulate different cellular responses, such as proliferation, differentiation, and apoptosis. It knows th…

  Identification and characterization of new phosphatases from the MAPK / ERK pathway, BP.DR

• Abstract

  FBXO25 protein, identified in silico in 1999, is one of 80 F-box proteins that serve as specificity factors for a family of ubiqutin ligases composed of Skp1, Rbx1, Cullin1 and an F-box protein (SCF1) that are involved in targeting proteins for destruction across the ubiquitin proteasome system. FBXO25 is able to form a functional SCF1 ubiquitin (Ub) ligase complex and FBXO25 protein acc…

  PRODUCTION AND FUNCTIONAL ANALYSIS OF HUMAN NEURONAL CELLS GENETICALLY MODIFIED TO REGULATE THE UBIQUITIN LIGASE FBXO25 EXPRESSION, BP.PD
• Application of protein microarray to identify substrates of SCF1 (FBXO25) E3 ubiquitin ligase, BP.PD

(Only some records are available in English at this moment)

• Abstract

  Fbxo7 is an unusual protein which has been found to be associated with two major human diseases, cancer and Parkinson's disease (PD). Fbxo7 is one of the 69 human F-box proteins that serve as specificity factors for the largest family of ubiquitin-ligases known as SCF-type E3 ubiquitin ligases. These modular, multi-subunited holoenzymes are composed of S-phase-kinase associated protein 1…

  Identification of the substrates of the E3 ubiquitin-ligase SCF(Fbxo7) using human proteome microarray, BE.EP.PD