Abstract
The Amblyomin-X is a Kunitz-type inhibitor that was identified in the transcriptome of Amblyomma cajennense through analysis of ESTs sequences from a cDNA library, was expressed with a 6xHis-Tag fused at its N-terminus in E. coli, representing a protein with 13.4 kDa, with a single single chain protein that contains seven cysteine residues. This protein is is able to inhibit FXa activit…