Rosana Puccia

• Abstract

  Paracoccidioides species and Histoplasma capsulatum are medically important thermodimorphic fungi and will be used as model systems in this project. The cell wall, which is the main fungal interface with the host, is constituted mainly of structural polysaccharides, specifically glucans, chitin, and galactomannan. This project aims to study, under different stress conditions, the global e…

  Expression and regulation of genes related to the cell wall from dimorphic pathogenic fungi under stress conditions, AP.R
• PCM 2011 - XI International Meeting on Paracoccidioidomycosis, AO.R

• Abstract

  We aim at using the fungal pathogens Paracoccidioides brasiliensis and Cryptococcus neoformans as model systems to the study of membranous vesicular structures (exosome-like) and of cell wall components. We intend to isolate and characterize the vesicles, to determine their composition in comparison with cell wall fractions, to evaluate their immunomodulatory potential and the role of mon…

  Analysis of exported vesicular structures and of secreted molecules by the pathogenic fungi Paracoccidioides brasiliensis e Cryptococcus neoformans, AP.TEM

(Only some records are available in English at this moment)

• Abstract

  We have previously shown in Paracoccidioides brasiliensis extracellular vesicles carrying immunogenic epitopes containing alfa-linked galactopyranosyl residues (alfa-Gal), which were also localized on the cell-wall and intracellular vacuoles of the fungal yeast cell. alfa-Gal epitopes produced intense reaction in ELISA with anti-alfa-Gal antibodies from chagasic and paracoccidioidomycosis…

  Characterization of alpha-galactopyranosyl epitopes in Paracoccidioides and other pathogenic fungal species, BP.DR

• Abstract

  Extracellular vesicles (EV) have recently been described in pathogenic fungi. In our laboratory, we have previously reported Paracoccidioides brasiliensis EV protein and lipid content, besides carbohydrate epitopes. Nowadays, we also have results of the RNA and polysaccharide EV content. Fungal EV carry components that potentially interact with the host, stimulate the immune system and a…

  Characterization of extracellular vesicles from pathogenic fungi under stress and their role in cellular communication, BP.DR

• Abstract

  Paracoccidioides species and Histoplasma capsulatum are medically important thermodimorphic fungi. The cell wall, which is the main interface of the fungus with the host, is constituted mainly of structural polysaccharides, which are remodelled during stress conditions. This project aims to study, under different stress conditions, the global expression regulation of transcription factors…

  Expression of genes related to the cell wall from dimorphic pathogenic fungi under stress conditions: identification of involved transcription factors, BP.PD

(Only some records are available in English at this moment)

• Abstract

  Histoplasma capsulatum is a medically important thermodimorphic fungal species in North and Latin America. The cell wall, which is the main interface of the fungus with the host, is composed mainly of structural polysaccharides, which are remodeled during stress conditions. Many fungal transcription factors have been involved in the response to different stress conditions, including Mns2/…

  Transcription factors and regulation of cell wall-related genes from Histoplasma capsulatum during stress, BE.EP.PD

• Abstract

  Molecules secreted to the cell surface or to the extracellular environment are obvious targets to participate in host-parasite interaction. Release of membranous vesicles is a conserved process in both prokaryote and eukaryote cells. Fungal extracellular vesicles (EV) are important structures involved in the transport of virulence factors including numerous proteins, neutral lipids, glyca…

  Terminal oligosaccharide profile of Paracoccidioides extracellular vesicle surface, BE.EP.DR