| Full text | |
| Author(s): |
Tambarussi, Evandro Vagner
[1]
;
Sebbenn, Alexandre Magno
[2]
;
Moreno, Maria Andreia
[1]
;
Ferraz, Elza Martins
[1]
;
Kageyama, Paulo Yoshio
[1]
;
Vencovsky, Roland
[1]
Total Authors: 6
|
| Affiliation: | [1] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, BR-13418900 Piracicaba, SP - Brazil
[2] Inst Florestal Sao Paulo, BR-01059970 Sao Paulo - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | APPLICATIONS IN PLANT SCIENCES; v. 1, n. 6 JUN 2013. |
| Web of Science Citations: | 8 |
| Abstract | |
Premise of the study: Microsatellite primers were developed in the neotropical tree species Cariniana legalis (Lecythidaceae) to investigate its genetic diversity, mating system, and gene flow. Methods and Results: We identified 96 clones containing 82 repeat motifs from a genomic library enriched for (CT)(8) and (GT)(8) motifs. Primer pairs were developed for 13 microsatellite loci and validated in 51 C. legalis specimens and 26 C. estrellensis specimens. Eleven loci were polymorphic, revealing a maximum of two to 15 alleles per locus in C. legalis and three to 12 in C. estrellensis. For C. legalis, the observed (H-o) and expected (H-e) heterozygosities ranged from 0 to 0.99 and from 0.07 to 0.90, respectively. For C. estrellensis, H-o and H-e ranged from 0 to 0.96 and from 0.14 to 0.91, respectively. Conclusions: The primers identified polymorphic loci that are suitable to study genetic diversity and structure, mating system, and gene flow in C. legalis and the related species C. estrellensis (AU) | |
| FAPESP's process: | 10/10704-7 - Contemporary gene flow, mating system, and spatial genetic structure in a jequitibá-rosa (Cariniana legalis Mart. Kuntze) fragmented population by microsatellite markers and parentage analysis |
| Grantee: | Alexandre Magno Sebbenn |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 10/12354-3 - Contemporary gene flow, mating system, and spatial genetic structure in a jequitibá-rosa (Cariniana legalis Mart. Kuntze) fragmented population by microsatellite markers. |
| Grantee: | Evandro Vagner Tambarussi |
| Support Opportunities: | Scholarships in Brazil - Doctorate |