PRIMERS FOR PHYLOGENY RECONSTRUCTION IN BIGNONIEAE (BIGNONIACEAE) USING HERBARIUM SAMPLES

Author(s)

Zuntini, Alexandre R. ^[1] ; Fonseca, Luiz Henrique M. ^[1] ; Lohmann, Lucia G. ^[1]

Affiliation

^[1] Univ Sao Paulo, Inst Biociencias, Dept Bot, BR-05508090 Sao Paulo - Brazil

• Document type: Journal article
• Source: APPLICATIONS IN PLANT SCIENCES; v. 1, n. 9 SEP 2013.
• Web of Science Citations: 9

Abstract

Premise of the study: New primers were developed for Bignonieae to enable phylogenetic studies within this clade using herbarium samples. Methods and Results: Internal primers were designed based on available sequences of the plastid ndhF gene and the rpl32-trnL intergenic spacer region, and the nuclear gene PepC. The resulting primers were used to amplify DNA extracted from herbarium materials. High-quality data were obtained from herbarium samples up to 53 yr old. Conclusions: The standardized methodology allows the inclusion of herbarium materials as alternative sources of DNA for phylogenetic studies in Bignonieae. (AU)

• FAPESP's process: 12/50260-6 - Structure and evolution of the Amazonian biota and its environment: an integrative approach
• Grantee: Lúcia Garcez Lohmann
• Support Opportunities: BIOTA-FAPESP Program - Thematic Grants
• FAPESP's process: 09/12508-3 - Molecular phylogeny of the genus Dolichandra s.l.
• Grantee: Luiz Henrique Martins Fonseca
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 11/50859-2 - Systematics of the tribe Bignonieae (Bignoniaceae)
• Grantee: Lúcia Garcez Lohmann
• Support Opportunities: Regular Research Grants
• FAPESP's process: 11/09160-5 - Taxonomic revision, phylogeny and biogeography of Bignonia L. (Bignonieae, Bignoniaceae)
• Grantee: Alexandre Rizzo Zuntini
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)