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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

PRIMERS FOR PHYLOGENY RECONSTRUCTION IN BIGNONIEAE (BIGNONIACEAE) USING HERBARIUM SAMPLES

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Author(s):
Zuntini, Alexandre R. [1] ; Fonseca, Luiz Henrique M. [1] ; Lohmann, Lucia G. [1]
Total Authors: 3
Affiliation:
[1] Univ Sao Paulo, Inst Biociencias, Dept Bot, BR-05508090 Sao Paulo - Brazil
Total Affiliations: 1
Document type: Journal article
Source: APPLICATIONS IN PLANT SCIENCES; v. 1, n. 9 SEP 2013.
Web of Science Citations: 10
Abstract

Premise of the study: New primers were developed for Bignonieae to enable phylogenetic studies within this clade using herbarium samples. Methods and Results: Internal primers were designed based on available sequences of the plastid ndhF gene and the rpl32-trnL intergenic spacer region, and the nuclear gene PepC. The resulting primers were used to amplify DNA extracted from herbarium materials. High-quality data were obtained from herbarium samples up to 53 yr old. Conclusions: The standardized methodology allows the inclusion of herbarium materials as alternative sources of DNA for phylogenetic studies in Bignonieae. (AU)

FAPESP's process: 09/12508-3 - Molecular phylogeny of the genus Dolichandra s.l.
Grantee:Luiz Henrique Martins Fonseca
Support type: Scholarships in Brazil - Master
FAPESP's process: 12/50260-6 - Structure and evolution of the Amazonian biota and its environment: an integrative approach
Grantee:Lúcia Garcez Lohmann
Support type: BIOTA-FAPESP Program - Thematic Grants
FAPESP's process: 11/50859-2 - Systematics of the tribe Bignonieae (Bignoniaceae)
Grantee:Lúcia Garcez Lohmann
Support type: Regular Research Grants
FAPESP's process: 11/09160-5 - Taxonomic revision, phylogeny and biogeography of Bignonia L. (Bignonieae, Bignoniaceae)
Grantee:Alexandre Rizzo Zuntini
Support type: Scholarships in Brazil - Doctorate (Direct)