| Full text | |
| Author(s): |
Ucci, Amanda P.
[1]
;
Martins, Paula M. M.
[2]
;
Lau, Ivy F.
[1]
;
Bacci, Jr., Mauricio
[2]
;
Belasque, Jr., Jose
[3]
;
Ferreira, Henrique
[1, 2]
Total Authors: 6
|
| Affiliation: | [1] Univ Estadual Paulista, Fac Ciencias Farmaceut, Dept Ciencias Biol, BR-14801902 Araraquara, SP - Brazil
[2] Univ Estadual Paulista, Inst Biociencias, Dept Bioquim & Microbiol, BR-13506900 Rio Claro, SP - Brazil
[3] Fundo Defesa Citricultura, BR-14807040 Araraquara, SP - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | MICROBIOLOGYOPEN; v. 3, n. 1, p. 29-41, FEB 2014. |
| Web of Science Citations: | 5 |
| Abstract | |
This study was intended to characterize the chromosome segregation process of Xanthomonas citri ssp. citri (Xac) by investigating the functionality of the ParB factor encoded on its chromosome, and its requirement for cell viability and virulence. Using TAP tagging we show that ParB is expressed in Xac. Disruption of parB increased the cell doubling time and precluded the ability of Xac to colonize the host citrus. Moreover, Xac mutant cells expressing only truncated forms of ParB exhibited the classical phenotype of aberrant chromosome organization, and seemed affected in cell division judged by their reduced growth rate and the propensity to form filaments. The ParB-GFP localization pattern in Xac was suggestive of an asymmetric mode of replicon partitioning, which together with the filamentation phenotype support the idea that Xac may control septum placement using mechanisms probably analogous to Caulobacter crescentus, and perhaps Vibrio cholerae, and Corynebacterium glutamicum. Xac exhibits asymmetric chromosome segregation, and the perturbation of this process leads to an inability to colonize the host plant. (AU) | |
| FAPESP's process: | 10/05099-7 - Chromosome segregation in Xanthomonas citri subsp. citri |
| Grantee: | Henrique Ferreira |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 10/02041-8 - Chromosome segregation in Xanthomonas citri subsp. citri |
| Grantee: | Amanda Piovesan Ucci |
| Support Opportunities: | Scholarships in Brazil - Doctorate |