Photochemical behavior of Safranine-Riboflavin com... - BV FAPESP
Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Photochemical behavior of Safranine-Riboflavin complex in the degradation of folic acid

Full text
Author(s):
Santos, Willy G. [1] ; Scurachio, Regina S. [1] ; Cardoso, Daniel R. [1]
Total Authors: 3
Affiliation:
[1] Univ Sao Paulo, Inst Quim Sao Carlos, BR-13560970 Sao Carlos, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY; v. 293, p. 32-39, NOV 1 2014.
Web of Science Citations: 9
Abstract

The photochemistry of safranine complexed with riboflavin and the photoinduced electron transfer from folic acid to the complex were investigated by steady-state and time resolved absorption spectroscopy, and the photoproducts were further analyzed by LC-orbitrap-FT-MS. UV-vis spectra of the complex showed a decrease in the safranine band at 520 nm with increasing concentration of riboflavin, which was attributed to complex formation. A 1:1 (safranine:riboflavin) stoichiometry was determined using Job's method. Following safranine photoexcitation, there is formation of a radical pair in the complex by donation of an electron from ground-state riboflavin to excited-state safranine. The cation radical of riboflavin within this complex reacts with folic acid to yield FA(+){*}, which may accept an electron from the anion radical of safranine, avoiding the photobleaching of the safranine. In contrast, the reaction of the anion radical of non-complexed safranine with folic acid leads to safranine photobleaching. (C) 2014 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 09/54040-8 - Acquisition of a high-resolution Orbitrap mass spectrometer for the discovery and structural analysis of biologically active compounds: applications in proteomics, biomarkers, synthesis, isolation, and characterization of natural products, studies of redox systems in food and enzymatic synthesis
Grantee:Emanuel Carrilho
Support Opportunities: Multi-user Equipment Program
FAPESP's process: 12/19823-4 - Phototransients of organoboranes generated by photolysis and Flavin sensitization.
Grantee:Willy Glen Santos
Support Opportunities: Scholarships in Brazil - Post-Doctoral