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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Evaluation of antibacterial activity of nitric oxide-releasing polymeric particles against Staphylococcus aureus and Escherichia coli from bovine mastitis

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Cardozo, Viviane F. [1] ; Lancheros, Cesar A. C. [1] ; Narciso, Adelia M. [2] ; Valereto, Elaine C. S. [2] ; Kobayashi, Renata K. T. [1] ; Seabra, Amedea B. [2] ; Nakazato, Gerson [1]
Total Authors: 7
[1] Univ Estadual Londrina, Dept Microbiol, BR-86055990 Londrina, PR - Brazil
[2] Univ Fed Sao Paulo, Exact & Earth Sci Dept, BR-09913030 Diadema, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: International Journal of Pharmaceutics; v. 473, n. 1-2, p. 20-29, OCT 1 2014.
Web of Science Citations: 37

Bovine mastitis is a serious veterinary disease that causes great loss to the dairy industry worldwide. It is a major infectious disease and is difficult to manage and control. Furthermore, emerging multidrug resistant bacteria that cause mastitis have complicated such management. The free radical nitric oxide (NO) is a potent antimicrobial agent. Thus, the aims of this study were to prepare and evaluate the antibacterial activity of nitric oxide-releasing polymeric particles against Staphylococcus aureus (MBSA) and Escherichia coli (MBEC), which were isolated from bovine mastitis. Fifteen MBSA isolates and fifteen MBEC were collected from subclinical and clinical bovine mastitis. Biocompatible polymeric particles composed of alginate/chitosan or chitosan/sodium tripolyphosphate (TPP) were prepared and used to encapsulate mercaptosuccinic acid (MSA), which is a thiol-containing molecule. Nitrosation of thiol groups of MSA-containing particles formed S-nitroso-MSA particles, which are NO donors. The NO release kinetics from the S-nitroso-MSA particles showed sustained and controlled NO release over several hours. The antibacterial activity of NO-releasing particles was evaluated by incubating the particles with an MBSA multi-resistant strain, which is responsible for bovine mastitis. The minimum inhibitory concentration for S-nitroso-MSA-alginate/chitosan particles against MBSA ranged from 125 mu g/mL to 250 mu g/mL. The results indicate that NO-releasing polymeric particles are an interesting approach to combating bacteria resistance in bovine mastitis treatment and prevention. (C) 2014 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 13/13096-6 - Nitric oxide releasing polymeric nanoparticles for antibacterial applications
Grantee:Adelia Maria Narciso
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 12/17053-7 - Nitric oxide releasing nanoparticles for biomedical applications
Grantee:Amedea Barozzi Seabra
Support Opportunities: Regular Research Grants