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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Nitrogen acquisition in Agave tequilana from degradation of endophytic bacteria

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Author(s):
Beltran-Garcia, Miguel J. [1, 2] ; White, Jr., James F. [3] ; Prado, Fernanda M. [1] ; Prieto, Katia R. [1] ; Yamaguchi, Lydia F. [4] ; Torres, Monica S. [3] ; Kato, Massuo J. [4] ; Medeiros, Marisa H. G. [1] ; Di Mascio, Paolo [1]
Total Authors: 9
Affiliation:
[1] Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-05508000 Sao Paulo - Brazil
[2] Univ Autonoma Guadalajara, Dept Quim ICET, Lomas Del Valle, Zapopan Jalisco - Mexico
[3] Rutgers State Univ, Dept Plant Biol & Pathol, New Brunswick, NJ 08903 - USA
[4] Univ Sao Paulo, Inst Quim, Dept Quim Fundamental, BR-05508000 Sao Paulo - Brazil
Total Affiliations: 4
Document type: Journal article
Source: SCIENTIFIC REPORTS; v. 4, NOV 6 2014.
Web of Science Citations: 18
Abstract

Plants form symbiotic associations with endophytic bacteria within tissues of leaves, stems, and roots. It is unclear whether or how plants obtain nitrogen from these endophytic bacteria. Here we present evidence showing nitrogen flow from endophytic bacteria to plants in a process that appears to involve oxidative degradation of bacteria. In our experiments we employed Agave tequilana and its seed-transmitted endophyte Bacillus tequilensis to elucidate organic nitrogen transfer from N-15-labeled bacteria to plants. Bacillus tequilensis cells grown in a minimal medium with (NH4Cl)-N-15 as the nitrogen source were watered onto plants growing in sand. We traced incorporation of N-15 into tryptophan, deoxynucleosides and pheophytin derived from chlorophyll a. Probes for hydrogen peroxide show its presence during degradation of bacteria in plant tissues, supporting involvement of reactive oxygen in the degradation process. In another experiment to assess nitrogen absorbed as a result of endophytic colonization of plants we demonstrated that endophytic bacteria potentially transfer more nitrogen to plants and stimulate greater biomass in plants than heat-killed bacteria that do not colonize plants but instead degrade in the soil. Findings presented here support the hypothesis that some plants under nutrient limitation may degrade and obtain nitrogen from endophytic microbes. (AU)

FAPESP's process: 12/12663-1 - Singlet molecular oxygen and peroxides in chemical biology
Grantee:Paolo Di Mascio
Support type: Research Projects - Thematic Grants
FAPESP's process: 11/10048-5 - Mapping adducts generated by endogenous and exogenous aldehydes: use as biomarkers of redox stress
Grantee:Marisa Helena Gennari de Medeiros
Support type: Research Projects - Thematic Grants
FAPESP's process: 13/07937-8 - Redoxome - Redox Processes in Biomedicine
Grantee:Ohara Augusto
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC