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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Maternal Western Style Diet Increases Susceptibility to Chemically-Induced Mammary Carcinogenesis in Female Rats Offspring

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Lopes, Gisele A. D. [1] ; Fan, William Y. C. [1] ; Ciol, Heloisa [2] ; Bidinotto, Lucas T. [3, 4] ; Rodrigues, Maria A. M. [1] ; Barbisan, Luis F. [2]
Total Authors: 6
[1] Sao Paulo State Univ, Botucatu Med Sch, Dept Pathol, Sao Paulo - Brazil
[2] Sao Paulo State Univ, Botucatu Biosci Inst, Dept Morphol, Sao Paulo - Brazil
[3] Barretos Canc Hosp, Mol Oncol Res Ctr, Sao Paulo - Brazil
[4] Dr Paulo Prata FACISB, Barretos Sch Hlth Sci, Sao Paulo - Brazil
Total Affiliations: 4
Document type: Journal article
Source: NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL; v. 66, n. 8, p. 1293-1303, NOV 17 2014.
Web of Science Citations: 2

The present study investigated whether maternal exposure to western style diet (WD) increases susceptibility to mammary carcinogenesis induced by N-methyl-N-nitrosourea (MNU) in female offspring. Pregnant female Sprague-Dawley rats received WD diet or control diet from gestational day 12 until postnatal day (PND) 21. At PND 21, female offspring received a single dose of MNU (50mg/kg body weight) and were fed chow diet until PND 110. Mammary gland structures were assessed on whole-mount preparations in the offspring at PND 21, and tumor morphology was examined at PND 110. Immunohistochemical analysis for cell proliferation (PCNA), apoptosis (cleaved caspase-3) and estrogen receptor alpha (ER-alpha) was performed in mammary terminal end buds (TEBs) at PND 21, and PCNA, ER-alpha, and p63 analysis in mammary tumors at PND 110. Maternal WD intake induced a significant increase in the number of TEBs (P = 0.024) and in PCNA labeling index (P < 0.020) in the mammary glands at PND 21. Tumor multiplicity, tumor weight, and PCNA labeling indexes were significantly higher in the WD offspring than that of the control offspring (P < 0.05). These findings indicate that maternal western style diet potentially enhanced the development of mammary tumors induced by MNU in female offspring, possibly by affecting the mammary gland differentiation. (AU)