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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Purification, crystallization and preliminary X-ray diffraction analysis of pyridoxal kinase from Plasmodium falciparum (PfPdxK)

Full text
Author(s):
Kronenberger, Thales [1] ; Lunev, Sergey [2] ; Wrenger, Carsten [1] ; Groves, Matthew R. [2]
Total Authors: 4
Affiliation:
[1] Univ Sao Paulo, Inst Biomed Sci, Dept Parasitol, Unit Drug Discovery, BR-05508000 Sao Paulo - Brazil
[2] Univ Groningen, Dept Drug Design, Groningen Res Inst Pharm GRIP, NL-9713 AV Groningen - Netherlands
Total Affiliations: 2
Document type: Journal article
Source: ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS; v. 70, n. 11, p. 1550-1555, NOV 2014.
Web of Science Citations: 1
Abstract

Pyridoxal kinases (PdxK) catalyze the phosphorylation of vitamin B-6 precursors. Thus, these enzymes are an essential part of many metabolic processes in all organisms. The protozoan parasite Plasmodium falciparum (the main causative agent of Malaria tropica) possesses a unique de novo B-6-biosynthesis pathway in addition to a interconversion pathway based on the activity of plasmodial PdxK (PfPdxK). The role of PdxK in B-6 salvage has prompted previous authors to suggest PdxK as a promising target for structure-based antimalarial drug design. Here, the expression, purification, crystallization and preliminary X-ray diffraction analysis of PfPdxK are reported. PfPdxK crystals have been grown in space group P2(1), with unit-cell parameters a = 52.7, b = 62.0, c = 93.7 angstrom, beta = 95 degrees. A data set has been collected to 2 angstrom resolution and an initial molecular-replacement solution is described. (AU)

FAPESP's process: 11/13706-3 - Implications from pyridoxine kinase of Plasmodium falciparum and study if its spacer regions
Grantee:Thales Kronenberger
Support type: Scholarships in Brazil - Master
FAPESP's process: 13/10288-1 - Analysis of the organelle biogenesis in Plasmodium falciparum by live cell imaging
Grantee:Carsten Wrenger
Support type: Regular Research Grants
FAPESP's process: 09/54325-2 - Elucidation of vitamin B metabolism in the human malaria parasite Plasmodium falciparum and their validation as a target for chemotherapy
Grantee:Carsten Wrenger
Support type: Research Grants - Young Investigators Grants