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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Antileishmanial and antitrypanosomal activity of the cutaneous secretion of Siphonops annulatus

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Author(s):
Pinto, Erika Gracielle [1, 2] ; Antoniazzi, Marta Maria [3] ; Jared, Carlos [3] ; Tempone, Andre Gustavo [2]
Total Authors: 4
Affiliation:
[1] Univ Sao Paulo, Sao Paulo Inst Trop Med, Sao Paulo - Brazil
[2] Adolfo Lutz Inst, Dept Parasitol & Micol, BR-01246000 Sao Paulo - Brazil
[3] Butantan Inst, Cell Biol Lab, Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Journal of Venomous Animals and Toxins including Tropical Diseases; v. 20, NOV 24 2014.
Web of Science Citations: 5
Abstract

Background: Among the tropical parasitic diseases, those caused by protozoans are considered a challenge to public health, being represented by leishmaniasis and Chagas disease. In view of the low effectiveness and toxicity of the current therapy, animal venoms such as amphibian secretions have been used as a promising source of new drug prototypes. The present work aimed to achieve bioguided fractionation of metabolites present in a cutaneous secretion of the caecilian Siphonops annulatus (Amphibia: Gymnophiona: Siphonopidae) with antileishmanial and antitrypanosomal activity. Methods: Through liquid-liquid partition and chromatographic techniques, the secretion was fractionated using bioguided assays. The 50% inhibitory concentration (IC50) of the main fraction (SaFr1) was studied against Leishmania (L.) infantum promastigotes and intracellular amastigotes, trypomastigotes of Trypanosoma cruzi and mammalian cells; viability was detected by the colorimetric MTT assay. By using a spectrofluorimetric assay with the probe SYTOX (R) Green and transmission electron microscopy (TEM), we also investigated the potential damage caused by SaFr1 in the plasma membrane and mitochondria of Leishmania. Results: The bioguided assay enabled isolation of a highly purified fraction (SaFr1) with an IC50 of 0.065 mu g/mL against promastigotes and 2.75 mu g/mL against trypomastigotes. Due to its high toxicity to peritoneal macrophages, SaFr1 showed no selectivity towards the intracellular forms of Leishmania. Ultrastructural studies with Leishmania demonstrated severe mitochondrial damage and the formation of large cytoplasmic vacuoles, leading to the parasite's death within a few hours. Nevertheless, it caused no alteration in the plasma membrane permeability as detected by the fluorescent probe and TEM. Conclusions: The present study demonstrated for the first time the antiparasitic activity of the skin secretion of the caecilian S. annulatus against Leishmania and T. cruzi, confirming that skin secretions of these amphibians, similarly to those of anurans and salamanders, are also potential tools for the development of new drug candidates against neglected diseases. (AU)

FAPESP's process: 09/12236-3 - Isolation, characterization, antileishmanial and antitrypanosomal activity of bioactive compounds from brazilian amphibian poisons
Grantee:Érika Gracielle Pinto
Support type: Scholarships in Brazil - Master
FAPESP's process: 08/09260-7 - Therapeutic combinations for visceral leishmaniasis: the antileishmanial potential of calcium channel blockers and the use of liposomal nanoformulations
Grantee:André Gustavo Tempone Cardoso
Support type: Regular Research Grants