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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

High genital prevalence of cutaneous human papillomavirus DNA on male genital skin: the HPV Infection in Men Study

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Author(s):
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Sichero, Laura [1] ; Campbell, Christine M. Pierce [2] ; Fulp, William [2] ; Ferreira, Silvaneide [1] ; Sobrinho, Joao S. [1] ; Baggio, Maria Luiza [1] ; Galan, Lenice [3] ; Silva, Roberto C. [4] ; Lazcano-Ponce, Eduardo [5] ; Giuliano, Anna R. [2] ; Villa, Luisa L. [1, 6, 7] ; Grp, HIM Study
Total Authors: 12
Affiliation:
[1] ICESP, Ctr Translat Oncol, Mol Biol Lab, BR-01246000 Sao Paulo - Brazil
[2] Univ S Florida, Coll Med, H Lee Moffitt Canc Ctr & Res Inst, Ctr Infect Res Canc, Tampa, FL 33612 - USA
[3] Ludwig Inst Canc Res, Sao Paulo - Brazil
[4] Ctr Referencia & Treinamento DST Aids, BR-04121000 Sao Paulo - Brazil
[5] Inst Nacl Salud Publ, Cuernavaca, Morelos - Mexico
[6] Univ Sao Paulo, Sch Med, Dept Radiol & Oncol, Santa Casa De Sao Paulo - Brazil
[7] HPV Inst, Santa Casa De Sao Paulo - Brazil
Total Affiliations: 7
Document type: Journal article
Source: BMC INFECTIOUS DISEASES; v. 14, DEC 9 2014.
Web of Science Citations: 15
Abstract

Background: The genital skin of males hosts a diversity of HPV genotypes and uncharacterized HPV genotypes. Previously we demonstrated that a specific viral genotype was not identified in 14% of all genital specimens (i.e., HPV unclassified specimens) using the Roche Linear Array method. Our goal was to identify and assess the prevalence of individual HPV types among genital HPV unclassified specimens collected in the HIM Study population, at enrollment, and examine associations with socio-demographic and behavioral characteristics. Methods: Genital skin specimens of men that were considered unclassified (HPV PCR positive, no genotype specified) at enrollment were typed by sequencing amplified PGMY09/11 products or cloning of PGMY/GP+ nested amplicons followed by sequencing. PGMY/GP+ negative specimens were further analyzed using FAP primers. HPV type classification was conducted through comparisons with sequences in the GenBank database. Results: Readable nucleotide sequences were generated for the majority of previously unclassified specimens (66%), including both characterized (77%) and yet uncharacterized (23%) HPV types. Of the characterized HPV types, most (73%) were Beta {[}beta]-HPVs, primarily from beta-1 and beta-2 species, followed by Alpha {[}alpha]-HPVs (20%). Smokers (current and former) were significantly more likely to have an alpha-HPV infection, compared with any other genus; no other factors were associated with specific HPV genera or specific beta-HPV species. Conclusions: Male genital skin harbor a large number of beta-HPV types. Knowledge concerning the prevalence of the diverse HPV types in the men genital is important to better understand the transmission of these viruses. (AU)

FAPESP's process: 08/57889-1 - Institute of Science and Technology to study Diseases Associated with Papillomavirus
Grantee:Luisa Lina Villa
Support type: Research Projects - Thematic Grants
FAPESP's process: 10/15282-3 - Diversity of HPV types in the male genital region
Grantee:Laura Cristina Sichero Vettorazzo
Support type: Regular Research Grants