Evaluation of methods of DNA extraction from Staphylococcus aureus in milk for use in real-time PCR

Author(s)

Dibbern, A. G. ^[1] ; Botaro, B. G. ^[1] ; Viziack, M. P. ^[1] ; Silva, L. F. P. ^[1] ; Santos, M. V. ^[1]

Affiliation

^[1] Univ Sao Paulo, Fac Med Vet & Zootecnia, Dept Nutr & Prod Anim, Pirassununga, SP - Brazil

• Document type: Journal article
• Source: Genetics and Molecular Research; v. 14, n. 1, p. 227-233, 2015.
• Web of Science Citations: 5

Abstract

The aim of this study was to evaluate the repeatability and performance of 4 methods of extracting DNA from Staphylococcus aureus (SAU) and the gene encoding bovine mitochondrial cytochrome B (BMCB) in milk samples from cows with subclinical mastitis for use in amplification by real-time polymerase chain reaction. Two milk samples were obtained from cows naturally infected with S. aureus and subjected to the following extraction methods: Qiagen DNA extraction kit; Axyprep DNA extraction kit; in silica column boil and in silica column method. After extraction in duplicate, eluates were subjected to purification and precipitation to determine purity (A(260)/A(280) ratio) and concentration (mu g/ mu L) by spectrophotometry and amplification by real-time polymerase chain reaction of target genes (SAU and BMCB). There was no effect of the DNA extraction method on DNA concentration and threshold cycle for BMCB and SAU. The purity ratio (A(260)/A(280)) was higher when using Qiagen DNA extraction (1.76 +/- 0.136) compared to the other methods tested. Our results indicate that the DNA extraction kit from Qiagen produces samples of the highest purity ratio compared to other methods. (AU)

• FAPESP's process: 10/10584-1 - Evaluation of four methods of DNA extraction and cattle for bacterial quantification by real time PCR
• Grantee: Aline Gerato Dibbern
• Support Opportunities: Scholarships in Brazil - Scientific Initiation