Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Phenotypic and genetic features of enteropathogenic Escherichia coli isolates from diarrheal children in the Ribeirao Preto metropolitan area, Sao Paulo State, Brazil

Full text
Author(s):
Pitondo-Silva, Andre [1] ; Nakazato, Gerson [2] ; Falcao, Juliana P. [1] ; Irino, Kinue [3] ; Martinez, Roberto [4] ; Darini, Ana Lucia C. [1] ; Hernandes, Rodrigo Tavanelli [5]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromat, Sao Paulo - Brazil
[2] Univ Estadual Londrina, Ctr Ciencias Biol, Dept Microbiol, Londrina, Parana - Brazil
[3] Adolfo Lutz Inst, Sao Paulo - Brazil
[4] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Clin Med, Sao Paulo - Brazil
[5] Univ Estadual Paulista, Inst Biociencias, Dept Microbiol & Imunol, Sao Paulo - Brazil
Total Affiliations: 5
Document type: Journal article
Source: APMIS; v. 123, n. 2, p. 128-135, FEB 2015.
Web of Science Citations: 8
Abstract

This study was designed to characterize a collection of 60 enteropathogenic Escherichia coli (EPEC) isolates from diarrheic feces of patients in the RibeirAo Preto metropolitan area regarding different phenotypic and molecular features. We examined antibiotic resistance profiles, occurrence of virulence factors-encoding genes, intimin subtypes and the correlation of serotypes among typical (tEPEC) and atypical (aEPEC) EPEC isolates. The results demonstrated that atypical EPEC was more heterogeneous than typical EPEC concerning the characteristics investigated and 45.2% do not belong to classical EPEC serogroups. Intimin subtype was the most frequent among the EPEC isolates (46.7%), being detected in both tEPEC and aEPEC. The majority of aEPEC isolates presented localized adherence-like (LAL) pattern to HEp-2 cells, although aEPEC isolates displaying diffuse adherence (DA) or non-adherent were also detected. High prevalence of antimicrobial resistance was found for ampicillin, cephalothin, sulfonamide and tetracycline. In general, tEPEC isolates were more resistant to the antimicrobials tested than aEPEC isolates. (AU)

FAPESP's process: 12/19077-0 - Prevalence and phenotypic and molecular characterization of Diarrheagenic Escherichia coli isolated from children with and without diarrhea in Botucatu, São Paulo
Grantee:Rodrigo Tavanelli Hernandes
Support Opportunities: Regular Research Grants