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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effect of pre-irradiation with different doses, wavelengths, and application intervals of low-level laser therapy on cytochrome c oxidase activity in intact skeletal muscle of rats

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Author(s):
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Albuquerque-Pontes, Gianna Moes [1] ; Vieira, Rodolfo de Paula [2, 3] ; Tomazoni, Shaiane Silva [4] ; Caires, Claudia Oliveira [1] ; Nemeth, Victoria [2] ; Vanin, Adriane Aver [2] ; Santos, Larissa Aline [2] ; Pinto, Henrique Dantas [2] ; Marcos, Rodrigo Labat [4] ; Bjordal, Jan Magnus [5, 6] ; Camillo de Carvalho, Paulo de Tarso [2, 1] ; Pinto Leal-Junior, Ernesto Cesar [2, 1]
Total Authors: 12
Affiliation:
[1] Univ Nove Julho UNINOVE, Postgrad Program Biophoton Appl Hlth Sci, BR-01504001 Sao Paulo - Brazil
[2] Univ Nove Julho UNINOVE, Postgrad Program Rehabil Sci, BR-01504001 Sao Paulo - Brazil
[3] Univ Nove Julho UNINOVE, Postgrad Program Med, BR-01504001 Sao Paulo - Brazil
[4] Univ Sao Paulo, Dept Pharmacol, Lab Pharmacol & Expt Therapeut, BR-05508900 Sao Paulo, SP - Brazil
[5] Univ Bergen, Fac Med & Dent, Dept Global Publ Hlth, Physiotherapy Res Grp, N-5020 Bergen - Norway
[6] Bergen Univ Coll, Ctr Knowledge Based Practice, N-5009 Bergen - Norway
Total Affiliations: 6
Document type: Journal article
Source: Lasers in Medical Science; v. 30, n. 1, p. 59-66, JAN 2015.
Web of Science Citations: 44
Abstract

Modulation of cytochrome c oxidase activity has been pointed as a possible key mechanism for low-level laser therapy (LLLT) in unhealthy biological tissues. But recent studies by our research group with LLLT in healthy muscles before exercise found delayed skeletal muscle fatigue development and improved biochemical status in muscle tissue. Therefore, the aim of this study was to evaluate effects of different LLLT doses and wavelengths in cytochrome c oxidase activity in intact skeletal muscle. In this animal experiment, we irradiated the tibialis anterior muscle of rats with three different LLLT doses (1, 3, and 10 J) and wavelengths (660, 830, and 905 nm) with 50 mW power output. After irradiation, the analyses of cytochrome c oxidase expression by immunohistochemistry were analyzed at 5, 10, 30 min and at 1, 2, 12, and 24 h. Our results show that LLLT increased (p < 0.05) cytochrome c oxidase expression mainly with the following wavelengths and doses: 660 nm with 1 J, 830 nm with 3 J, and 905 nm with 1 J at all time points. We conclude that LLLT can increase cytochrome c oxidase activity in intact skeletal muscle and that it contributes to our understanding of how LLLT can enhance performance and protect skeletal muscles against fatigue development and tissue damage. Our findings also lead us to think that the combined use of different wavelengths at the same time can enhance LLLT effects in skeletal muscle performance and other conditions, and it can represent a therapeutic advantage in clinical settings. (AU)

FAPESP's process: 12/06832-5 - Low-level laser therapy and topical sodium diclofenac in contusion-induced skeletal muscle injury in rats
Grantee:Lúcio Frigo
Support type: Regular Research Grants
FAPESP's process: 12/11385-8 - Effect of different doses, wavelengths and application intervals of low-level laser therapy in cytochrome c-oxidase activity in different types of muscle fibers of skeletal muscle in rats.
Grantee:Gianna Móes Albuquerque Pontes
Support type: Scholarships in Brazil - Master
FAPESP's process: 10/52404-0 - Low-level laser therapy in skeletal muscle fatigue and post-exercise recovery: Optimal parameters and effects in long-duration exercise
Grantee:Ernesto Cesar Pinto Leal Junior
Support type: Research Grants - Young Investigators Grants