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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Prenatal zinc reduces stress response in adult rat offspring exposed to lipopolysaccharide during gestation

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Galvao, Marcella C. [1] ; Chaves-Kirsten, Gabriela P. [1] ; Queiroz-Hazarbassanov, Nicolle [1] ; Carvalho, Virginia M. [1] ; Bernardi, Maria M. [2] ; Kirsten, Thiago B. [1, 2]
Total Authors: 6
[1] Univ Sao Paulo, Sch Vet Med, Dept Pathol, BR-05508270 Sao Paulo - Brazil
[2] Univ Paulista, BR-04026002 Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Life Sciences; v. 120, p. 54-60, JAN 1 2015.
Web of Science Citations: 8

Aims: Previous investigations by our group have shown that prenatal treatment with lipopolysaccharide (LPS; 100 mu g/kg, intraperitoneally) on gestation day (GD) 9.5 in rats, which mimics infections by Gram-negative bacteria, induces short-and long-term behavioral and neuroimmune changes in the offspring. Because LPS induces hypozincemia, dams were treated with zinc after LPS in an attempt to prevent or ameliorate the impairments induced by prenatal LPS exposure. LPS can also interfere with hypothalamic-pituitary-adrenal (HPA) axis development; thus, behavioral and neuroendocrine parameters linked to HPA axis were evaluated in adult offspring after a restraint stress session. Main methods: We prenatally exposed Wistar rats to LPS (100 mu g/kg, intraperitoneally, on GD 9.5). One hour later they received zinc (ZnSO4, 2 mg/kg, subcutaneously). Adult female offspring that were in metestrus/diestrus were submitted to a 2 h restraint stress session. Immediately after the stressor, 22 kHz ultrasonic vocalizations, open field behavior, serum corticosterone and brain-derived neurotrophic factor (BDNF) levels, and striatal and hypothalamic neurotransmitter and metabolite levels were assessed. Key findings: Offspring that received prenatal zinc after LPS presented longer periods in silence, increased locomotion, and reduced serum corticosterone and striatal norepinephrine turnover compared with rats treated with LPS and saline. Prenatal zinc reduced acute restraint stress response in adult rats prenatally exposed to LPS. Significance: Our findings suggest a potential beneficial effect of prenatal zinc, in which the stress response was reduced in offspring that were stricken with infectious/inflammatory processes during gestation. (C) 2014 Elsevier Inc. All rights reserved. (AU)

FAPESP's process: 12/07007-8 - Possible prevention or treatment of impairments induced by an early prenatal exposure to lipopolysaccharide in an experimental model of autism
Grantee:Thiago Berti Kirsten
Support type: Scholarships in Brazil - Post-Doctorate