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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Antimicrobial photodynamic therapy against pathogenic bacterial suspensions and biofilms using chloro-aluminum phthalocyanine encapsulated in nanoemulsions

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Dias Ribeiro, Ana Paula [1] ; Andrade, Mariana Carvalho [2] ; Bagnato, Vanderlei Salvador [3] ; Vergani, Carlos Eduardo [2] ; Primo, Fernando Lucas [4] ; Tedesco, Antnio Claudio [2, 4] ; Pavarina, Ana Claudia [5]
Total Authors: 7
[1] Univ Brasilia UnB, Sch Hlth Sci, Dept Dent, Campus Darcy Ribeiro, DF - Brazil
[2] Univ Estadual Paulista, UNESP, Araraquara Dent Sch, Dept Dent Mat & Prosthodont, BR-14801903 Araraquara, SP - Brazil
[3] Univ Sao Paulo, Phys Inst Sao Carlos, Sao Carlos, SP - Brazil
[4] Univ Sao Paulo, Photobiol & Photomed Res Grp, Ctr Nanotechnol & Tissue Engineers, FFCLRP, Ribeirao Preto, SP - Brazil
[5] Univ Estadual Paulista, UNESP, Fac Odontol Araraquara, BR-14801903 Araraquara, SP - Brazil
Total Affiliations: 5
Document type: Journal article
Source: Lasers in Medical Science; v. 30, n. 2, p. 549-559, FEB 2015.
Web of Science Citations: 13

Antimicrobial photodynamic therapy represents an alternative method of killing resistant pathogens. Efforts have been made to develop delivery systems for hydrophobic drugs to improve the photokilling. This study evaluated the photodynamic effect of chloro-aluminum phthalocyanine (ClAlPc) encapsulated in nanoemulsions (NE) on methicillin-susceptible and methicillin-resistant Staphylococcus aureus suspensions and biofilms. Suspensions and biofilms were treated with different delivery systems containing ClAlPc. After the pre-incubation period, the drug was washed-out and irradiation was performed with LED source (660 +/- 3 nm). Negative control samples were not exposed to ClAlPc or light. For the suspensions, colonies were counted (colony-forming units per milliliter (CFU/mL)). The metabolic activity of S. aureus suspensions and biofilms were evaluated by the XTT assay. The efficiency was dependent on the delivery system, superficial load and light dose. Cationic NE-ClAlPc and free-ClAlPc caused photokilling of the both strains of S. aureus. For biofilms, cationic NE-ClAlPc reduced cell metabolism by 80 and 73 % of susceptible and resistant strains, respectively. Although anionic NE-ClAlPc caused a significant CFU/ml reduction for MSSA and MRSA, it was not capable of reducing MRSA biofilm metabolism. This therapy may represent an alternative treatment for eradicating resistant strains. (AU)

FAPESP's process: 09/17975-9 - Effectiveness of antimicrobial photodynamic therapy on Candida inactivation and the cellular damage caused to epithelial cells. In vitro and in vivo evaluation of aluminum-chloro-phthalocyanine and other derivates.
Grantee:Ana Paula Dias Ribeiro
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 10/05425-1 - Evaluation of the effectiveness of antimicrobial photodynamic therapy in the inactivation of Candida species and cellular damage caused to skin cells: in vitro and in vivo evaluation of the aluminum-chloro-phtalocyanine and other derivatives
Grantee:Ana Cláudia Pavarina
Support Opportunities: Regular Research Grants