| Full text | |
| Author(s): |
Pozzi, Fernanda S.
[1]
;
Maranhao, Raul C.
[1, 2]
;
Guedes, Lissiane K.
[3]
;
Borba, Eduardo F.
[3]
;
Laurindo, Ieda M. M.
[3]
;
Bonfa, Eloisa
[3]
;
Vinagre, Carmen G.
[1]
Total Authors: 7
|
| Affiliation: | [1] Univ Sao Paulo, Med Sch Hosp, Inst Heart, Lipid Metab Lab, Sao Paulo - Brazil
[2] Univ Sao Paulo, Dept Clin Biochem, Fac Pharmaceut Sci, Sao Paulo - Brazil
[3] Univ Sao Paulo, Med Sch Hosp, Div Rheumatol, Sao Paulo - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | JOURNAL OF CLINICAL LIPIDOLOGY; v. 9, n. 1, p. 72-80, FEB 2015. |
| Web of Science Citations: | 3 |
| Abstract | |
BACKGROUND: Rheumatoid arthritis (RA) is a systemic inflammatory disease associated with cardiovascular risk, but with normal plasma lipids. OBJECTIVE: The aim was to investigate low-density lipoprotein (LDL) and high-density lipoprotein (HDL) metabolism in RA patients using radioactive nanoemulsions resembling an LDL lipid structure (LDE) as metabolic probes. METHODS: Thirty patients with RA, 16 in remission and 14 in high activity, and 30 healthy controls were studied. LDE labeled with C-14-cholesteryl ester (C-14-CE) and H-3-unesterified cholesterol (H-3-UC) was intravenously injected followed by 24 hour plasma sampling. Fractional clearance rates (FCR, h(-1)) were calculated by compartmental analysis. Lipid transfers to HDL were assayed by incubating plasma samples with a donor nanoemulsion labeled with radioactive lipids; % lipids transferred to HDL were quantified after chemical precipitation. RESULTS: LDL cholesterol, triglycerides, unesterified cholesterol, and oxidized LDL were equal in RA and controls, and HDL cholesterol was even higher in RA. Compared with controls, apolipoprotein B was lower, apolipoprotein A1 was equal, and apolipoprotein E was higher in RA. Decay curves of LDE labels were faster in RA patients than in controls (C-14-CE: 0.072 +/- 0.066 and 0.038 +/- 0.027, P = .0115; H-3-UC: 0.066 +/- 0.042 and 0.035 +/- 0.039; P < .0044). FCRs were equal in 2 RA subgroups. Transfer of UC, triglycerides, and phospholipids to HDL was equal between RA and controls, but CE transfer was lower in RA. HDL size was smaller in RA patients than in controls (8.5 +/- 0.5 nm; 9.2 +/- 0.8 nm, P < .0001). CONCLUSION: RA patients were more efficient in removing atherogenic LDL from plasma, as indicated by higher CE and UC FCR, with in lower apolipoprotein B. This was unexpected because of the higher cardiovascular risk in RA. (C) 2015 National Lipid Association. All rights reserved. (AU) | |
| FAPESP's process: | 13/05293-6 - A new therapeutic approach in cardiology: treatment of acute myocardial infarction with methotrexate associated with lipid nanoemulsions |
| Grantee: | Raul Cavalcante Maranhao |
| Support Opportunities: | Regular Research Grants |