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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Association between Cationic Liposomes and Low Molecular Weight Hyaluronic Acid

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Author(s):
Gasperini, Antonio A. M. [1] ; Puentes-Martinez, Ximena E. [1, 2] ; Balbino, Tiago Albertini [3] ; Rigoletto, Thais de Paula [4] ; Cavalcanti Correa, Gabriela de Sa [3] ; Cassago, Alexandre [5] ; Portugal, Rodrigo Villares [5] ; de La Torre, Lucimara Gaziola [3] ; Cavalcanti, Leide P. [1]
Total Authors: 9
Affiliation:
[1] CNPEM, Brazilian Synchrotron Light Lab, BR-13083970 Campinas, SP - Brazil
[2] Univ Fed Sao Paulo, Inst Ciencia & Tecnol, BR-12231280 Sao Paulo - Brazil
[3] Univ Estadual Campinas, Sch Chem Engn, BR-13083970 Campinas, SP - Brazil
[4] Ctr Univ Fac Associadas Ensino UNIFAE, BR-13870377 Sao Paulo - Brazil
[5] CNPEM, Brazilian Nanotechnol Natl Lab, BR-13083970 Campinas, SP - Brazil
Total Affiliations: 5
Document type: Journal article
Source: Langmuir; v. 31, n. 11, p. 3308-3317, MAR 24 2015.
Web of Science Citations: 12
Abstract

This work presents a study of the association between low molecular weight hyaluronic acid (16 kDa HA) and cationic liposomes composed of egg phosphatidylcholine (EPC), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), and 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP). The cationic liposome/HA complexes were evaluated to determine their mesoscopic structure, average size, zeta potential, and morphology as a function of the amount of HA in the system. Small angle X-ray scattering results revealed that neighboring cationic liposomes either stick together after a partial coating of low concentration HA or disperse completely in excess of HA, but they never assemble as multilamellar vesicles. Cryo-transmission electron microscopy images confirm the existence of unilamellar vesicles and large aggregates of unilamellar vesicles for HA fractions up to 80% (w/w). High concentrations of HA (> 20% w/w) proved to be efficient for coating extruded liposomes, leading to particle complexes with sizes in the nanoscale range and a negative zeta potential. (AU)

FAPESP's process: 11/19952-6 - STRUCTURAL CHARACTERIZATION OF AMPHIPHILIC SYSTEMS WITH INDUSTRIAL APPLICATION USING SMALL ANGLE X RAY SCATTERING AND COMPLEMENTARY TECHNIQUES
Grantee:Antonio Augusto Malfatti Gasperini
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 10/02203-8 - Development of cationic liposomes shielded by hyaluronic acid for DNA vaccination against leishmaniasis
Grantee:Lucimara Gaziola de la Torre
Support Opportunities: Regular Research Grants