| Full text | |
| Author(s): |
Alvarenga, Leticia Heineck
[1]
;
Prates, Renato Araujo
[2, 3]
;
Yoshimura, Tania Mateus
[4]
;
Kato, Ilka Tiemy
[5]
;
Suzuki, Luis Claudio
[4]
;
Ribeiro, Martha Simoes
[4]
;
Ferreira, Luis Rodolfo
[2, 3]
;
dos Santos Pereira, Silvio Antonio
[1]
;
Martinez, Elizabeth Ferreira
[1]
;
Saba-Chujfi, Eduardo
[1]
Total Authors: 10
|
| Affiliation: | [1] Sao Leopoldo Mandic, Dent Res Ctr, Campinas - Brazil
[2] Nove Julho Univ UNINOVE, Postgrad Program Biophoton Appl Hlth Sci, BR-01504001 Sao Paulo, SP - Brazil
[3] Nove Julho Univ UNINOVE, Sch Dent, BR-01504001 Sao Paulo, SP - Brazil
[4] Nucl & Energy Res Inst IPEN CNEN SP, Ctr Lasers & Applicat, Sao Paulo - Brazil
[5] ABC Fed Univ UFABC, Dept Biomed Engn, Santo Andre, SP - Brazil
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | Photodiagnosis and Photodynamic Therapy; v. 12, n. 1, p. 131-135, MAR 2015. |
| Web of Science Citations: | 16 |
| Abstract | |
Background: The purpose of this study was to evaluate the antibacterial effects of photodynamic action of methylene blue (MB) against Aggregatibacter actinomycetemcomitans organized on biofilm. Methods: After the biofilm growth in 96 flat-bottom well plate, the following groups were used: control group, untreated by either laser or photosensitizer (PS); MB group or dark toxicity group, which was exposed to MB atone (100 mu M) for 1 min (pre-irradiation time); laser group, irradiated with laser for 5 min in the absence of PS and three antimicrobial photodynamic inactivation (APDI) groups, with three exposure times of 1, 3 and 5 min of irradiation, corresponding to fluences of 15, 45, and 75 J/cm(2) respectively. The results were compared to the control group for statistical proposes. Scanning electronic microscope analysis was used to access structural changes in biofilm. Results: Red laser alone and MB alone were not able to inactivate bacterial biofilm. APDI groups showed differences when compared to the control group and they were dependent on the exposure time. No statistically significant differences were observed among the APDI groups at 1 and 3 min of irradiation. On the other hand, 5 min of APDI showed 99.85% of bacterial reduction (p=0.0004). In addition, the biofilm loose its structure following 5 min APDI. Conclusions: The results of this study suggest that A. actinomycetemcomitans biofilm can be inactivated by MB mediated APDI. (C) 2014 Elsevier B.V. All rights reserved. (AU) | |
| FAPESP's process: | 05/01756-5 - Estudo dos efeitos da terapia fotodinamica antimicrobiana em periodontite induzida em ratos. |
| Grantee: | Martha Simões Ribeiro |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 10/13313-9 - DEVELOPMENT OF METHODOLOGIES FOR PHOTODYNAMIC THERAPY APPLICATIONS ON FUNGAL INFECTIONS |
| Grantee: | Martha Simões Ribeiro |
| Support Opportunities: | Regular Research Grants |