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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Chitin-degrading enzymes from an actinomycete ectosymbiont of Acromyrmex subterraneus brunneus (Hymenoptera: Formicidae)

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Author(s):
Rossi, Guilherme Duarte [1, 2] ; Zucchi, Tiago Domingues [3, 1] ; Guidolin, Aline Sartori [1] ; Peruchi, Aline [1] ; Consoli, Fernando Luis [1]
Total Authors: 5
Affiliation:
[1] Univ Sao Paulo, ESALQ, Dept Entomol & Acarol, Lab Interacoes Insetos, Piracicaba, SP - Brazil
[2] UNESP, FCAV, Dept Fitossanidade, Jaboticabal, SP - Brazil
[3] Embrapa Meio Ambiente, Lab Microbiol Ambiental, Jaguariuna, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: ANNALS OF MICROBIOLOGY; v. 65, n. 1, p. 565-574, MAR 2015.
Web of Science Citations: 0
Abstract

Microbes have many mechanisms to exert their inhibitory activity against target pests. One such mechanism involves the production and secretion of hydrolytic enzymes, such as chitinases, which are produced naturally by plants in response to attack by insect herbivores and phytopathogens and have been sought as an additional factor to enhance pest management. Thus, our main aim was to screen the diverse actinomycete community associated with the integument of Acromyrmex subterraneus brunneus for a chitinase-producing strain and to characterize its chitinases. We identified isolate ENT-21-a Streptomyces sp.-as a chitinase-producer and our data indicate that this isolate produces a chitinolytic complex that contains a chitinase and a high-molecular-weight beta-N-acetylglucosaminidase (> 100 kDa) when cultured in Chitin-Czapek broth. The presence of chitinases in the genome of this isolate was checked by diagnostic PCR, and two chitinase genes belonging to family 18 group A and family 19 were verified. The chitinolytic activity of the crude extract was observed at pH values ranging from 3.8 to 11.0, with the highest chitinase activities recorded at pH 9.0 and 9.5, whereas optimum beta-N-acetylglucosaminidase activity was observed over a narrow pH range, between pH 4.7 and 5.1. We describe some biochemical and molecular properties of the chitinase and beta-N-acetylglucosaminidase produced by ENT-21, and discuss the potential for exploitation of these enzymes for pest control. (AU)

FAPESP's process: 11/50877-0 - Diversity, ecology and biotechnological potential of the symbiotic bacteriofauna associated with insects
Grantee:Fernando Luis Cônsoli
Support type: BIOTA-FAPESP Program - Thematic Grants
FAPESP's process: 11/14333-6 - Diversity and polyphasic taxonomy of actinobacteria isolated from reforestation areas
Grantee:Tiago Domingues Zucchi
Support type: Scholarships in Brazil - BIOTA - Young Researchers
FAPESP's process: 10/13675-8 - Characterization and bioindustrial application of the gut bacteriofauna associated with the termites Armitermes euamignathus Silvestri, 1901 (Isoptera: Termitidae: Syntermitinae) e Heterotermes tenuis (Hagen, 1858) (Isoptera: Rhinotermitidae)
Grantee:Aline Peruchi
Support type: Scholarships in Brazil - Master
FAPESP's process: 12/04287-0 - Toxoptera citricida (Kirkaldy) (Hemiptera: Aphidae): associated simbionts and interactions with secondary symbionts
Grantee:Aline Sartori Guidolin
Support type: Scholarships in Brazil - Doctorate