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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

DNA Methylation Levels of Melanoma Risk Genes Are Associated with Clinical Characteristics of Melanoma Patients

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de Araujo, Erica S. S. [1] ; Pramio, Dimitrius T. [1] ; Kashiwabara, Andre Y. [2] ; Pennacchi, Paula C. [3] ; Maria-Engler, Silvya S. [3] ; Achatz, Maria I. [1, 4] ; Campos, Antonio H. J. F. M. [5] ; Duprat, Joao P. [6] ; Rosenberg, Carla [7] ; Carraro, Dirce M. [1] ; Krepischi, Ana C. V. [1, 7]
Total Authors: 11
[1] AC Camargo Canc Ctr, Int Res Ctr, BR-01508010 Sao Paulo, SP - Brazil
[2] Fed Technol Univ Parana, BR-86300000 Cornelio Procopio, PR - Brazil
[3] Univ Sao Paulo, Sch Pharmaceut Sci, BR-05508000 Sao Paulo, SP - Brazil
[4] AC Camargo Canc Ctr, Dept Oncogenet, BR-01509010 Sao Paulo, SP - Brazil
[5] AC Camargo Canc Ctr, Dept Pathol, BR-01509010 Sao Paulo, SP - Brazil
[6] AC Camargo Canc Ctr, Skin Canc Dept, BR-01509010 Sao Paulo, SP - Brazil
[7] Univ Sao Paulo, Inst Biosci, Dept Genet & Evolutionary Biol, BR-05508090 Sao Paulo, SP - Brazil
Total Affiliations: 7
Document type: Journal article
Web of Science Citations: 4

In melanoma development, oncogenic process is mediated by genetic and epigenetic mutations, and few studies have so far explored the role of DNA methylation either as predisposition factor or biomarker. We tested patient samples for germline CDKN2A methylation status and found no evidence of inactivation by promoter hypermethylation. We have also investigated the association of clinical characteristics of samples with the DNA methylation pattern of twelve genes relevant for melanomagenesis. Five genes (BAP1, MGMT, MITF, PALB2, and POT1) presented statistical association between blood DNA methylation levels and either CDKN2A-mutation status, number of lesions, or Breslow thickness. In tumors, five genes (KIT, MGMT, MITF, TERT, and TNF) exhibited methylation levels significantly different between tumor groups including acral compared to nonacral melanomas and matched primary lesions and metastases. Our data pinpoint that the methylation level of eight melanoma-associated genes could potentially represent markers for this disease both in peripheral blood and in tumor samples. (AU)

FAPESP's process: 13/07480-8 - Genetic and epigenetic factors in the etiology of the cutaneous melanoma
Grantee:Ana Cristina Victorino Krepischi
Support type: Regular Research Grants
FAPESP's process: 13/10785-5 - Investigation of mutational profile ín cutaneous melanoma ánd impact ón “The global DNA methylation pattern
Grantee:Dimitrius Tansini Pramio
Support type: Scholarships in Brazil - Master
FAPESP's process: 12/13963-9 - Global genomic methylation profile in familial melanoma syndrome
Grantee:Érica Sara Souza de Araújo
Support type: Scholarships in Brazil - Doctorate