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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The Effect of Essential Oils and Bioactive Fractions on Streptococcus mutans and Candida albicans Biofilms: A Confocal Analysis

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Author(s):
Freires, Irlan Almeida [1] ; Bueno-Silva, Bruno [2] ; de Carvalho Galvao, Livia Camara [1] ; Teixeira Duarte, Marta Cristina [3] ; Sartoratto, Adilson [3] ; Figueira, Glyn Mara [3] ; de Alencar, Severino Matias [4] ; Rosalen, Pedro Luiz [1]
Total Authors: 8
Affiliation:
[1] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Physiol Sci, Pharmacol Anesthesiol & Therapeut, BR-13414903 Piracicaba, SP - Brazil
[2] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, BR-05508900 Sao Paulo, SP - Brazil
[3] Univ Estadual Campinas, Res Ctr Chem Biol & Agr, BR-13083970 Campinas, SP - Brazil
[4] Univ Sao Paulo, Luiz de Queiroz Coll Agr, Dept Agrifood Ind Food & Nutr, BR-13414903 Piracicaba, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Evidence-based Complementary and Alternative Medicine; 2015.
Web of Science Citations: 6
Abstract

The essential oils (EO) and bioactive fractions (BF) from Aloysia gratissima, Baccharis dracunculifolia, Coriandrum sativum, Cyperus articulatus, and Lippia sidoides were proven to have strong antimicrobial activity on planktonic microorganisms; however, little is known about their effects on the morphology or viability of oral biofilms. Previously, we determined the EO/fractions with the best antimicrobial activity against Streptococcus mutans and Candida spp. In this report, we used a confocal analysis to investigate the effect of these EO and BF on the morphology of S. mutans biofilms (thickness, biovolume, and architecture) and on the metabolic viability of C. albicans biofilms. The analysis of intact treated S. mutans biofilms showed no statistical difference for thickness in all groups compared to the control. However, a significant reduction in the biovolume of extracellular polysaccharides and bacteria was observed for A. gratissima and L. sidoides groups, indicating that these BF disrupt biofilm integrity and may have created porosity in the biofilm. This phenomenon could potentially result in a weakened structure and affect biofilm dynamics. Finally, C. sativum EO drastically affected C. albicans viability when compared to the control. These results highlight the promising antimicrobial activity of these plant species and support future translational research on the treatment of dental caries and oral candidiasis. (AU)

FAPESP's process: 11/14757-0 - Evaluation of antimicrobial activity of essential oils on oral pathogens
Grantee:Pedro Luiz Rosalen
Support type: Regular Research Grants
FAPESP's process: 12/01500-4 - Assessment of effects of propolis and its bioactive compounds on the modulation of of macrophages inflammatory response
Grantee:Bruno Bueno Silva
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 11/15984-0 - Anti-Candida, antiproliferative and cytotoxic activities evaluation of essential oil and fractions from Coriandrum sativum L.
Grantee:Irlan de Almeida Freires
Support type: Scholarships in Brazil - Master
FAPESP's process: 09/12353-0 - Evaluation of antimicrobial activity of essential oils from plants against mutans microorganisms
Grantee:Lívia Câmara de Carvalho Galvão
Support type: Scholarships in Brazil - Master