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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Interleukin-18 Increases TLR4 and Mannose Receptor Expression and Modulates Cytokine Production in Human Monocytes

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Dias-Melicio, Luciane Alarcao [1] ; Fernandes, Reginaldo Keller [2] ; Rodrigues, Daniela Ramos [2] ; Golim, Marjorie Assis [3] ; Victoriano Campos Soares, Angela Maria [2]
Total Authors: 5
[1] Univ Estadual Paulista UNESP, Fac Med, Dept Patol, BR-18618970 Botucatu, SP - Brazil
[2] Univ Estadual Paulista UNESP, Inst Biociencias, Dept Microbiol & Imunol, BR-18618970 Botucatu, SP - Brazil
[3] Univ Estadual Paulista UNESP, Fac Med, BR-18618970 Botucatu, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Mediators of Inflammation; 2015.
Web of Science Citations: 10

Interleukin-18 is a proinflammatory cytokine belonging to the interleukin-1 family of cytokines. This cytokine exerts many unique biological and immunological effects. To explore the role of IL-18 in inflammatory innate immune responses, we investigated its impact on expression of two toll-like receptors (TLR2 and TLR4) and mannose receptor (MR) by human peripheral blood monocytes and its effect on TNF-alpha, IL-12, IL-15, and IL-10 production. Monocytes from healthy donors were stimulated or not with IL-18 for 18 h, and then the TLR2, TLR4, and MR expression and intracellular TNF-alpha, IL-12, and IL-10 production were assessed by flow cytometry and the levels of TNF-alpha, IL-12, IL-15, and IL-10 in culture supernatants were measured by ELISA. IL-18 treatment was able to increase TLR4 and MR expression by monocytes. The production of TNF-alpha and IL-10 was also increased by cytokine treatment. However, IL-18 was unable to induce neither IL-12 nor IL-15 production by these cells. Taken together, these results show an important role of IL-18 on the early phase of inflammatory response by promoting the expression of some pattern recognition receptors (PRRs) that are important during the microbe recognition phase and by inducing some important cytokines such as TNF-alpha and IL-10. (AU)

FAPESP's process: 07/04042-9 - Role of IL-18 on the interaction between human monocytes and Paracoccidioides Brasiliensis: modulation of TLR2 and TLR4, induction of fungicidal activity and modulation of oxidative metabolism
Grantee:Angela Maria Victoriano de Campos Soares
Support type: Regular Research Grants