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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effect of Helicobacter pylori Eradication on TLR2 and TLR4 Expression in Patients with Gastric Lesions

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Targa Cadamuro, Aline Cristina [1] ; Teixeira Rossi, Ana Flavia [1] ; Biselli-Perico, Joice Matos [1] ; Pereira, Patricia Fucuta [2] ; Bedin Mascarin Do Vale, Edla Polsinelli [2] ; Acayaba, Ricardo [3] ; Moreira Leite, Ktia Ramos [4] ; Goloni-Bertollo, Eny Maria [5] ; Silva, Ana Elizabete [1]
Total Authors: 9
[1] Sao Paulo State Univ UNESP, Dept Biol, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil
[2] Ambulatory Gastrohepatol, Base Hosp, BR-15090000 Sao Jose Do Rio Preto, SP - Brazil
[3] Joao Paulo II Hosp, BR-15030070 Sao Jose Do Rio Preto, SP - Brazil
[4] Univ Sao Paulo, Sch Med, BR-01246903 Sao Paulo, SP - Brazil
[5] FAMERP, Sch Med, BR-15090000 Sao Jose Do Rio Preto, SP - Brazil
Total Affiliations: 5
Document type: Journal article
Source: Mediators of Inflammation; 2015.
Web of Science Citations: 5

Objective. Helicobacter pylori (Hp) is recognized by TLR4 and TLR2 receptors, which trigger the activation of genes involved in the host immune response. Thus, we evaluated the effect of eradication therapy on TLR2 and TLR4 mRNA and protein expression in H. pylori-infected chronic gastritis patients (CG-Hp+) and 3 months after treatment. Methods. A total of 37 patients CG-Hp+ were evaluated. The relative quantification (RQ) of mRNA was assessed by TaqMan assay and protein expression by immunohistochemistry. Results. Before treatment both TLR2 and TLR4 mRNA in CG-Hp+ patients were slightly increased (TLR2 = 1.32; TLR4 = 1.26) in relation to Hp-negative normal gastric mucosa (P <= 0.05). After successful eradication therapy no significant change was observed (TLR2 = 1.47; TLR4 = 1.53; P > 0.05). In addition, the cagA and vacA bacterial genotypes did not influence the gene expression levels, and we observed a positive correlation between the RQ values of TLR2 and TLR4, both before and after treatment. Immunoexpression of the TLR2 and TLR4 proteins confirmed the gene expression results. Conclusion. In conclusion, the expression of both TLR2 and TLR4 is increased in CG-Hp+ patients regardless of cagA and vacA status and this expression pattern is not significantly changed after eradication of bacteria, at least for the short period of time evaluated. (AU)

FAPESP's process: 12/15036-8 - Evaluation of gene and protein expression in inflammatory processes and neoplasms of the digestive tract: stomach and colorectal
Grantee:Ana Elizabete Silva
Support type: Regular Research Grants