Glucose Biochip Based on Flexible Carbon Fiber Electrodes: In Vivo Diabetes Evaluation in Rats

Author(s)

Iost, Rodrigo M. ^[1] ; Sales, Fernanda C. P. F. ^[1] ; Martins, Marccus V. A. ^[2] ; Almeida, Maria C. ^[2] ; Crespilho, Frank N. ^[1]

Affiliation

^[1] Univ Sao Paulo, Inst Quim Sao Carlos, BR-13560970 Sao Carlos, SP - Brazil

^[2] Univ Fed ABC, Ctr Ciencias Nat & Humanas, BR-09210170 Santo Andre, SP - Brazil

• Document type: Journal article
• Source: CHEMELECTROCHEM; v. 2, n. 4, p. 518-521, APR 15 2015.
• Web of Science Citations: 6

Abstract

This paper reports an implantable biochip for intravenous glucose monitoring in rat blood. A key feature of this device is the combination of the flexibility of the flexible carbon fiber (FCF) electrodes for the micromanipulation of the biosensor in the implant. FCF, modified with a neutral red electrochemical mediator and an enzyme glucose oxidase, enables the biochip to be manipulated conveniently during insertion in the rat veins. For invivo experiments, the biochip is inserted into the jugular vein of a living rat (Rattus novergicus) using a poly(propylene) catheter. The capability for invivo glucose detection is evaluated with a normal concentration of glucose (30mgdL(-1)) and with a diabetic simulation (200mgdL(-1)). Through electrochemical data, this type of biochip can distinguish different concentration of glucose in the blood of the animal. The biochip showed promise performance for future applications of implantable bioelectronics devices. (AU)

• FAPESP's process: 13/04663-4 - Enzymatic bioelectrodes applied in biofuel cells: experimental and simulation
• Grantee: Frank Nelson Crespilho
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/14262-7 - Nanostructured films from biologically-relevant materials
• Grantee: Osvaldo Novais de Oliveira Junior
• Support Opportunities: Research Projects - Thematic Grants