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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Resistance Training Regulates Cardiac Function through Modulation of miRNA-214

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Soares Melo, Stephano Freitas [1] ; Barauna, Valerio Garrone [2] ; Carneiro Junior, Miguel Araujo [3] ; Marchesi Bozi, Luiz Henrique [1] ; Drummond, Lucas Rios [3] ; Natali, Antonio Jose [3] ; de Oliveira, Edilamar Menezes [1]
Total Authors: 7
[1] Univ Sao Paulo, Lab Biochem & Mol Biol Exercise, Sch Phys Educ & Sport, BR-05508030 Sao Paulo - Brazil
[2] Univ Fed Espirito Santo, Hlth Sci Ctr, Lab Mol Physiol, BR-29043900 Vitoria - Brazil
[3] Univ Fed Vicosa, Dept Phys Educ, BR-36570900 Vicosa, MG - Brazil
Total Affiliations: 3
Document type: Journal article
Source: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 16, n. 4, p. 6855-6867, APR 2015.
Web of Science Citations: 18

Aims: To determine the effects of resistance training (RT) on the expression of microRNA (miRNA)-214 and its target in sarcoplasmic reticulum Ca2+-ATPase (SERCA2a), and on the morphological and mechanical properties of isolated left ventricular myocytes. Main methods: Male Wistar rats were divided into two groups (n = 7/group): Control (CO) or trained (TR). The exercise-training protocol consisted of: 4 x 12 bouts, 5x/week during 8 weeks, with 80% of one repetition maximum. Key findings: RT increased the left ventricular myocyte width by 15% and volume by 12%, compared with control animals (p < 0.05). The time to half relaxation and time to peak were 8.4% and 4.4% lower, respectively, in cells from TR group as compared to CO group (p < 0.05). RT decreased miRNA-214 level by 18.5% while its target SERCA2a expression were 18.5% higher (p < 0.05). Significance: Our findings showed that RT increases single left ventricular myocyte dimensions and also leads to faster cell contraction and relaxation. These mechanical adaptations may be related to the augmented expression of SERCA2a which, in turn, may be associated with the epigenetic modification of decreased miRNA-214 expression. (AU)

FAPESP's process: 09/18370-3 - MicroRNA expression on normotensive rats heart submitted to different aerobic training and the potential to hypertension therapy
Grantee:Edilamar Menezes de Oliveira
Support type: Regular Research Grants
FAPESP's process: 10/09438-0 - Therapeutic use of microRNA responsible for regulating cardiac contractility associated with exercise training aerobic in rats with heart failure
Grantee:Stéphano Freitas Soares Melo
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 10/50048-1 - Cellular and functional bases of exercise in cardiovascular diseases
Grantee:Carlos Eduardo Negrão
Support type: Research Projects - Thematic Grants