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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Immediate and late analysis of dental pulp stem cells viability after indirect exposition to alternative in-office bleaching strategies

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Soares, Diana Gabriela [1] ; Basso, Fernanda Goncalves [1] ; Hebling, Josimeri [2] ; de Souza Costa, Carlos Alberto [1]
Total Authors: 4
[1] Univ Estadual Paulista UNESP, Dept Physiol & Pathol, Araraquara Sch Dent, BR-14801903 Araraquara, SP - Brazil
[2] Univ Estadual Paulista UNESP, Dept Orthodont & Pediat Dent, Araraquara Sch Dent, BR-14801903 Araraquara, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: CLINICAL ORAL INVESTIGATIONS; v. 19, n. 5, p. 1013-1020, JUN 2015.
Web of Science Citations: 15

Objectives To evaluate human dental pulp stem cell viability and capacity to recover from experimental dental bleaching techniques. Material and methods Enamel/dentin disks adapted to trans-wells were positioned on previously cultivated dental pulp stem cells. Bleaching gels containing 35, 17.5, 10, and 8 % hydrogen peroxide (H2O2) were applied one or three times (each application lasting 15 min) on enamel. Cell viability (MTT assay) and morphology (SEM) were evaluated immediately (T1) or 72 h (T2) post-bleaching. Results The 35 % H2O2 gel promoted intense reduction in viability (93-97 %) and morphological alterations of the cells at T1, irrespective of frequency of application, with absence or limited capacity for recovery being observed at T2. The other bleaching gels presented significant lower toxicity when compared with the 35% H2O2 gel, in a time/concentration fashion. In T1, no significant difference was observed between the negative control (without bleaching) and the 8 and 10 % H2O2 gels applied on enamel for 15 min, in which the cells presented elevated viability and morphology similar to the negative control at T2. Conclusions Bleaching gels with 8 and 10 % H2O2 in their composition cause limited immediate toxic effect on pulp stem cells, which recover their viability 3 days after treatment. Clinical relevance This study presents proposals for in-office dental bleaching to be performed with limited aggressive effect on dental pulp stem cells. Therefore, we are able to offer interesting clinical alternatives for bleaching vital teeth, under professional supervision, maintaining the integrity and reparative capacity of pulp-dentin complex. (AU)

FAPESP's process: 11/12938-8 - New parameters for tooth bleaching: an evaluation of efficacy, citotoxicity and molecular effects
Grantee:Carlos Alberto de Souza Costa
Support Opportunities: Regular Research Grants
FAPESP's process: 11/09385-7 - New parameters for tooth bleaching: an evaluation of efficacy, citotoxicity and molecular effects
Grantee:Diana Gabriela Soares dos Passos
Support Opportunities: Scholarships in Brazil - Doctorate